R
Rocio Bartolome-Cabrero
Researcher at Spanish National Research Council
Publications - 6
Citations - 289
Rocio Bartolome-Cabrero is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Lipase & Immobilized enzyme. The author has an hindex of 4, co-authored 5 publications receiving 207 citations.
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Journal ArticleDOI
Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase
Sara Peirce,Sara Peirce,Jose J. Virgen-Ortíz,Veymar G. Tacias-Pascacio,Nazzoly Rueda,Nazzoly Rueda,Rocio Bartolome-Cabrero,Laura Fernandez-Lopez,Maria Russo,Antonio Marzocchella,Roberto Fernandez-Lafuente +10 more
TL;DR: The proposed protocol permitted the reuse of the most stable enzyme after inactivation of the least stable one, and is compatible with any immobilization protocol of the first enzyme that does not involve ion exchange as only reason for enzyme immobilization.
Journal ArticleDOI
Stabilizing effects of cations on lipases depend on the immobilization protocol
Laura Fernandez-Lopez,Rocio Bartolome-Cabrero,María Daniela Rodríguez,María Daniela Rodríguez,Cleiton S. dos Santos,Cleiton S. dos Santos,Nazzoly Rueda,Nazzoly Rueda,Roberto Fernandez-Lafuente +8 more
TL;DR: This is the first report of a cation induced enzyme stabilization effect that depends on the lipase immobilization protocol, and seems to be based on a specific mechanism, and required the recognition sites to be saturated by the cations.
Journal ArticleDOI
Improved immobilization and stabilization of lipase from Rhizomucor miehei on octyl-glyoxyl agarose beads by using CaCl2
Laura Fernandez-Lopez,Nazzoly Rueda,Nazzoly Rueda,Rocio Bartolome-Cabrero,María Daniela Rodríguez,María Daniela Rodríguez,Tiago Lima de Albuquerque,Tiago Lima de Albuquerque,José C. S. dos Santos,José C. S. dos Santos,Oveimar Barbosa,Roberto Fernandez-Lafuente +11 more
TL;DR: A strategy to stabilize octyl (OC)- Rhizomucor miehei lipase (RML) and OC- Candida rugosa lipase at pH 10 is necessary to take full advantage of the immobilization of these enzymes on OC-glyoxyl ( OCGLX) supports.
Journal ArticleDOI
Reversible Immobilization of Lipases on Heterofunctional Octyl-Amino Agarose Beads Prevents Enzyme Desorption
Nazzoly Rueda,Nazzoly Rueda,Tiago Lima de Albuquerque,Tiago Lima de Albuquerque,Rocio Bartolome-Cabrero,Laura Fernandez-Lopez,Rodrigo Torres,Claudia Ortiz,José C. S. dos Santos,José C. S. dos Santos,Oveimar Barbosa,Roberto Fernandez-Lafuente +11 more
TL;DR: Thermal and solvent stabilities of LU and TLL were significantly improved compared to the OC counterparts, while in the other enzymes the stability decreased in most cases (depending on the pH value).
Book ChapterDOI
Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons.
TL;DR: In this paper, the authors outline two methods to obtain either mouse or human neurons derived from postnatal astrocytes and skin fibroblasts, respectively, based on the co-expression of Bcl-2 and the transcription factor Neurog2 that produces mostly excitatory neurons.