Roman Fisher

TL;DR: A new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics.

TL;DR: It is demonstrated that normal T cell biology is critically dependent on CCT-controlled proteostasis and that its absence is incompatible with protective immunity.

TL;DR: This article used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, skin, and plasma of mice across the life course and reported highly dynamic matrisome turnover in bone and cartilage during skeletal maturation which was markedly reduced after skeletal maturity.

TL;DR: In this article, the presence of known interactors of KRAS including a-RAF and LZTR1, which varied in abundance with wild type and KRAS mutants, was demonstrated by quantitative proteomics.

TL;DR: In this paper, the authors describe a label-free proteomics method suitable for small cardiac tissue biopsies based on density-separated fractionation, which allows study of endo-lysosomal (EL) proteins.