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Romy Kandzia
Researcher at Princeton University
Publications - 14
Citations - 4153
Romy Kandzia is an academic researcher from Princeton University. The author has contributed to research in topics: Molecular cloning & Follicular lymphoma. The author has an hindex of 8, co-authored 14 publications receiving 3536 citations.
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A one pot, one step, precision cloning method with high throughput capability.
TL;DR: A cloning strategy called ‘Golden Gate’ cloning was devised that allows to obtain in one tube and one step close to one hundred percent correct recombinant plasmids after just a 5 minute restriction-ligation, thus providing precision for this fundamental process of genetic manipulation.
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Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.
TL;DR: It is shown that one round of shuffling using the 27trypsinogen entry plasmids can easily produce the 19,683 different possible combinations in one single restriction-ligation and that expression screening of a subset of the library allows identification of variants that can lead to higher expression levels of trypsin activity.
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Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants.
TL;DR: It is shown here that Agrobacterium–mediated delivery of constructs for the efficient delivery of RNA viral vectors as DNA precursors results in gene amplification in all mature leaves of a plant simultaneously (systemic transfection).
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In planta engineering of viral RNA replicons: Efficient assembly by recombination of DNA modules delivered by Agrobacterium
TL;DR: The proposed protocol allows a plant with a mixture of two or more agrobacteria carrying specific prefabricated modules to rapidly and inexpensively assemble and test multiple vector/gene combinations, without the need to perform the various engineering steps normally required with alternative protocols.
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High-yield production of authentic human growth hormone using a plant virus-based expression system
TL;DR: The results indicate that the use of viral vectors for high-yield production of human therapeutic proteins in plants by transient expression provides an attractive alternative to production protocols using standard expression vectors in transgenic or transplastomic plants.