Ronald D. Wesley

TL;DR: Replicating kinetoplast DNA networks from both Crithidia fasciculata and Leishmania tarentolae have an equilibrium density in ethidium bromide—CsCl which is less than that of covalently closed non-replicating networks, implying that there is some type of lateral mobility of molecules within the network, by which minicircles move past the peripheral locus of replication.

TL;DR: Quantitative renaturation kinetic studies of purified kinetoplast minicircular DNA from Leishmania tarentolae indicated that there is probably one but certainly no more than two classes of identically sized minicircles in terms of DNA base sequences.

TL;DR: Purified kinetoplast DNA minicircles from Leishmania tarentolae promastigotes have been studied in terms of several physical properties: contour length and molecular weight, superhelix density of covalently closed molecules, buoyant density in CsCl, sedimentation coefficients, and thermal melting characteristics.

TL;DR: Two basic techniques for the recovery of kinetoplast DNA minicircles have been developed which have increased the understanding of the nature of the mitochondrial genome in the hemoflagellate, Leishmania tarentolae.