So far in this course we have dealt entirely with the evolution of characters that are controlled by simple Mendelian inheritance at a single locus. There are notes on the course website about gametic disequilibrium and how allele frequencies change at two loci simultaneously, but we didn’t discuss them. In every example we’ve considered we’ve imagined that we could understand something about evolution by examining the evolution of a single gene. That’s the domain of classical population genetics. For the next few weeks we’re going to be exploring a field that’s actually older than classical population genetics, although the approach we’ll be taking to it involves the use of population genetic machinery. If you know a little about the history of evolutionary biology, you may know that after the rediscovery of Mendel’s work in 1900 there was a heated debate between the “biometricians” (e.g., Galton and Pearson) and the “Mendelians” (e.g., de Vries, Correns, Bateson, and Morgan). Biometricians asserted that the really important variation in evolution didn’t follow Mendelian rules. Height, weight, skin color, and similar traits seemed to

Human biochemical genetics

The terms 'antioxidant', 'oxidative stress' and 'oxidative damage' are widely used but rarely defined. This brief review attempts to define them and to examine the ways in which oxidative stress and oxidative damage can affect cell behaviour both in vivo and in cell culture, using cancer as an example.

Biochemistry of oxidative stress

ECOLOGY/EVOLUTION: Phenotypic Plasticity

When the red harvester ant is threatened, nestmates rush to its assistance. The message is a chemical one. Distress is communicated and help summoned by the potential victim ejecting a tiny cocktail of alkanes and terpenoids. Professor E 0 Wilson, who made this discovery, is a distinguished zoologist whose writings have twice been awarded Pulitzer Prizes. His interests extend far beyond entomology, however. Why should human communication be qualitatively different from that of the ant, in being achieved by definable, physicochemical processes? And, if this is the case, surely the same must be true of other social phenomena? Having conceded so much, on what grounds can we exclude other products of human activity such as the social sciences, arts, humanities and ethics? In a mechanistic universe, the whole of human civilization is the ultimate product of the interaction between genes and environment, incredibly complex, as Wilson admits, but susceptible to fundamentally the same analytic techniques as communication in the harvester ant. Consilience (literally, a 'jumping together') is the notion that a common groundwork of explanation applies not only to the sciences but also to the arts, ethics and religion. In his book Consiliencel Wilson applies a combination of broad learning and a style of baroque exuberance to put the case for such a unity of knowledge. Wilson is thus, as he makes clear, a child of the Enlightenment, paying homage to Bacon and Newton but most of all to Condorcet, who trod the same intellectual path (and who came to grief in the Revolution for reasons probably not unrelated to his philosophy). He mourns the triumph at the end of the eighteenth century of revelatory religion fearful of the outcomes of science, and most of all of Romanticism although regrettably overlooking the

Consilience: The Unity of Knowledge.

Unchecked aggression and violence exact a significant toll on human societies. Aggression is an umbrella term for behaviours that are intended to inflict harm. These behaviours evolved as adaptations to deal with competition, but when expressed out of context, they can have destructive consequences. Uncontrolled aggression has several components, such as impaired recognition of social cues and enhanced impulsivity. Molecular approaches to the study of aggression have revealed biological signals that mediate the components of aggressive behaviour. These signals may provide targets for therapeutic intervention for individuals with extreme aggressive outbursts. This Review summarizes the complex interactions between genes, biological signals, neural circuits and the environment that influence the development and expression of aggressive behaviour.

Neural mechanisms of aggression

Snarr, RL, Esco, MR, Tolusso, DV, Hallmark, AV, Earley, RL, Higginbotham, JC, Fedewa, MV, and Bishop, P. Comparison of lactate and electromyographical thresholds after an exercise bout. J Strength Cond Res 33(12): 3322-3331, 2019-The electromyographical threshold (EMGT) has been previously validated as a means to predict the work rate at which lactate threshold (LT) occurs. The reliability of these measures has yet to be examined after a bout of exercise. The purpose was to determine the agreement between electromyography (EMG) and LT after a 30-minute bout of steady-state aerobic exercise. Participants completed 2 graded exercise tests (GXT) on a cycle ergometer separated by 30 minutes of steady-state exercise. Blood lactate was measured the last 45 seconds of each stage during both GXTs, whereas EMG of the vastus lateralis was monitored continuously. Individual agreement demonstrated that pre-exercise and post-exercise LT occurred at the same work rate in 2 of 10 participants, whereas EMGT occurred at the same work rate in 6 of 10 participants. Results showed no mean difference between work rates for LT or EMG threshold for the pre-exercise GXT, but LT was significantly lower (p < 0.01) than EMGT during the post-exercise GXT. Post-GXT LT work rates were also determined to be significantly lower than pre-GXT LT (p = 0.034), whereas no differences existed in EMG thresholds. Although both LT and EMGT testing may display similar properties, they are not interchangeable. The physiological responses to increasing exercise intensity between La and EMG signaling seem to be associated, and their interaction may not be cause-effect. Because of poor individual agreement, caution should be used when determining LT through the use of EMG. Further research is needed to determine the ability of these 2 metrics to prescribe training intensities.

Comparison of Lactate and Electromyographical Thresholds After an Exercise Bout.

Synopsis The adaptive significance of colorful or exaggerated traits (i.e., ornaments) expressed in females is often unclear. Competing hypotheses suggest that expression of female ornaments arises from maladaptive (or neutral) genetic inheritance from males along with incomplete epigenetic regulation, or from positive selection for ornaments in females under social competition. Whether costly or advantageous, the visibility of such traits can sometimes be behaviorally modulated in order to maximize fitness. Female eastern fence lizards express blue badges that are variable in size and color saturation. These are rudimentary compared to those seen in males and carry important costs such as reduced mating opportunities. Body temperature is a well-established enhancer of badge color, and thus thermoregulation may be one way these animals modulate badge visibility. We quantified realized body temperatures of female lizards paired in laboratory trials and observed that females with larger badges attained higher body temperatures when freely allowed to thermoregulate, sometimes beyond physiological optima. In this association between phenotype and behavior, females with larger badges exhibited thermoregulatory patterns that increase their badges’ visibility. This signal-enhancing behavior is difficult to reconcile with the widely held view that female ornaments are maladaptive, suggesting they may carry context-dependent social benefits.

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Masculinized Sexual Ornaments in Female Lizards Correlate with Ornament-Enhancing Thermoregulatory Behavior

Fighting experiences strongly influence aggressive behavior and physiology (winner-loser effects). These effects are conserved from invertebrates to vertebrates, but the underlying mechanisms remain unclear. Recent studies indicate that the brain social decision-making network (SDN) plays a key role in guiding experience-induced behavioral change. Also, while most studies have focused on how winning and losing experiences alter aggression, growing evidence points to these experiences driving multiple behavioral effects, including changes in the ability to learn. In mangrove rivulus fish (Kryptolebias marmoratus), we discovered that single winning experiences significantly improved spatial learning but not risk-avoidance learning, whereas single losing experiences drove the exact opposite to occur. These results provide strong evidence that winning and losing modulate diverse behaviors served by key nodes within the SDN, specifically the dorsolateral pallium (Dl; fish homolog to mammalian hippocampus, which serves spatial learning) and dorsomedial pallium (Dm; fish homolog to mammalian basolateral amygdala, which responds to fear). We therefore quantified whole-proteome expression within the forebrain (where Dm and Dl are located) of adult rivulus with divergent social experiences. We discovered 23 proteins were significantly differentially expressed in the forebrains of winners and losers. Differentially expressed proteins in losers related to modulation of cellular processes, apoptosis and learning while those in winners related to neuronal plasticity, neuroendocrine homeostasis, energy utilization, and learning. These results imply that winner-loser effects might be governed by very different patterns of protein expression, which could explain why winners and losers show such pronounced differences in behavioral performance. Significance StatementSocial interactions permeate the daily lives of most animals and often result in changes in behavior for all parties. This implies that social experiences reorganize the brain in ways that promote the expression of alternative behaviors, or that help individuals cope with the outcome of such interactions. But how do aggressive interactions sculpt the brain at the molecular level? We used an emerging model organism, Kryptolebias marmoratus, to examine whether experiences modulate learning ability and then probe the potential neural mechanisms underlying these behavioral changes. We discovered that single winning and losing experiences dramatically altered spatial learning and risk-avoidance learning, respectively, indicating that winning and losing experiences have markedly different effects on the brain and cognitive processes. ClassificationBiological Sciences, Ecology

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Social experience alters different types of learning abilities controlled by distinct brain nuclei in Kryptolebias marmoratus

ii DEDICATION iii LIST OF ABBREVIATIONS AND SYMBOLS iv ACKNOWLEDGMENTS v LIST OF TABLES viii LIST OF FIGURES ix

The taste of aggression: a model for psychopathy and reactive aggression

Passive dispersal via wind or ocean currents can drive asymmetric gene flow, which influences patterns of genetic variation and the capacity of populations to evolve in response to environmental change. The mangrove rivulus fish (Kryptolebias marmoratus), hereafter “rivulus,” is an intertidal fish species restricted to the highly fragmented New World mangrove forests of Central America, the Caribbean, the Bahamas, and Florida. Mangrove patches are biological islands with dramatic differences in both abiotic and biotic conditions compared to adjacent habitat. Over 1,000 individual rivulus across 17 populations throughout its range were genotyped at 32 highly polymorphic microsatellites. Range-wide population genetic structure was evaluated with five complementary approaches that found eight distinct population clusters. However, an analysis of molecular variance indicated significant population genetic structure among regions, populations within regions, sampling locations within populations, and individuals within sampling locations, indicating that rivulus has both broad- and fine-scale genetic differentiation. Integrating range-wide genetic data with biophysical modeling based on 10 years of ocean current data showed that ocean currents and the distance between populations over water drive gene flow patterns on broad scales. Directional migration estimates suggested some significant asymmetries in gene flow that also were mediated by ocean currents and distance. Specifically, populations in the center of the range (Florida Keys) were identified as sinks that received migrants (and alleles) from other populations but failed to export individuals. These populations thus harbor genetic variation, perhaps even from extirpated populations across the range, but ocean currents and complex arrangements of landmasses might prevent the distribution of that genetic variation elsewhere. Hence, the inherent asymmetry of ocean currents shown to impact both genetic differentiation and directional migration rates may be responsible for the complex distribution of genetic variation across the range and observed patterns of metapopulation structure.

/pdf/out-to-sea-ocean-currents-and-patterns-of-asymmetric-gene-3tf1nhey.pdf

Ryan L. Earley

Papers

Comparison of Lactate and Electromyographical Thresholds After an Exercise Bout.

Masculinized Sexual Ornaments in Female Lizards Correlate with Ornament-Enhancing Thermoregulatory Behavior

Social experience alters different types of learning abilities controlled by distinct brain nuclei in Kryptolebias marmoratus

The taste of aggression: a model for psychopathy and reactive aggression

Out to sea: ocean currents and patterns of asymmetric gene flow in an intertidal fish species