S. Kriminski

TL;DR: A fixed-temperature annealing protocol for reducing flash-cooling-induced disorder of macromolecular crystals is demonstrated that should be more reliable and flexible than existing protocols.

TL;DR: Direct fluorescence imaging of the three‐dimensional impurity distribution shows that impurities incorporate with different densities in sectors formed by growth on different crystal faces, and that impurity density in the crystal core and along boundaries between growth sectors can be much larger than in other parts of the crystal.

TL;DR: Factors affecting the success of flash-cooling experiments can be ordered from most to least important as follows: crystal solvent content and solvent composition, crystal size and shape, amount of residual liquid around the crystal, cooling method (liquid plunge versus gas stream), choice of gas/liquid and relative speed between cooling fluid and crystal.

TL;DR: In this article, the second-order elastic moduli of tetragonal hen egg-white lysozyme crystals were determined as a function of relative humidity (RH) by Brillouin scattering.

TL;DR: Brillouin linewidths can be described by a mechanical model in which the phonon is coupled to a relaxation mode of hydration water with a single relaxation time of 55 +/- 5 ps and constraints on the elastic tensor are obtained by combining the Brillouin quasilongitudinal results with axial compressibilities determined from high-pressure x-ray diffraction.