S
Sabine Zitzenbacher
Researcher at University of Natural Resources and Life Sciences, Vienna
Publications - 30
Citations - 1022
Sabine Zitzenbacher is an academic researcher from University of Natural Resources and Life Sciences, Vienna. The author has contributed to research in topics: Sialyltransferase & Cutinase. The author has an hindex of 14, co-authored 28 publications receiving 697 citations. Previous affiliations of Sabine Zitzenbacher include Hoffmann-La Roche.
Papers
More filters
Journal ArticleDOI
Enhanced Cutinase-Catalyzed Hydrolysis of Polyethylene Terephthalate by Covalent Fusion to Hydrophobins
Doris Ribitsch,Enrique Herrero Acero,Agnieszka Przylucka,Sabine Zitzenbacher,Annemarie Marold,Caroline Gamerith,Rupert Tscheließnig,Alois Jungbauer,Harald Rennhofer,Helga C. Lichtenegger,Heinz Amenitsch,Klaus Bonazza,Christian P. Kubicek,Irina S. Druzhinina,Georg M. Guebitz +14 more
TL;DR: The data show that the level of hydrolysis of PET by cutinase can be significantly increased by fusion to hydrophobins, and suggest that this likely involves binding of the hydphobins to the cutin enzyme and changes in the conformation of its active center.
Journal ArticleDOI
Fusion of binding domains to Thermobifida cellulosilytica cutinase to tune sorption characteristics and enhancing PET hydrolysis.
Doris Ribitsch,Antonio Orcal Yebra,Sabine Zitzenbacher,Jing Wu,Susanne Nowitsch,Georg Steinkellner,Katrin Greimel,Aleš Doliška,Gustav Oberdorfer,Christian Gruber,Karl Gruber,Helmut Schwab,Karin Stana-Kleinschek,Enrique Herrero Acero,Georg M. Guebitz +14 more
TL;DR: Increased adsorption to PET by the fusion enzymes was confirmed with Quarz Crystal Microbalance (QCM-D) analysis and indeed resulted in enhanced hydrolysis activity (3.8× for Thc_Cut1+CBM) on PET, as quantified, based on released mono/oligomers.
Journal ArticleDOI
A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET) and Polylactic Acid (PLA)
Doris Ribitsch,Enrique Herrero Acero,Katrin Greimel,Anita Dellacher,Sabine Zitzenbacher,Annemarie Marold,Rosario Diaz Rodriguez,Georg Steinkellner,Karl Gruber,Helmut Schwab,Georg M. Guebitz +10 more
TL;DR: A new esterase from Thermobifida halotolerans (Thh_Est) was cloned and expressed in E. coli and investigated for surface hydrolysis of polylactic acid (PLA) and polyethylene terephthalate (PET) as discussed by the authors.
Journal ArticleDOI
Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis.
Enrique Herrero Acero,Doris Ribitsch,Anita Dellacher,Sabine Zitzenbacher,Annemarie Marold,Georg Steinkellner,Karl Gruber,Helmut Schwab,Georg M. Guebitz +8 more
TL;DR: Findings clearly demonstrate that surface properties (i.e., amino acids located outside the active site on the protein surface) play an important role in PET hydrolysis.
Journal ArticleDOI
Improving enzymatic polyurethane hydrolysis by tuning enzyme sorption
Caroline Gamerith,Enrique Herrero Acero,Alessandro Pellis,Andreas Ortner,Robert Vielnascher,Daniel Luschnig,Barbara Zartl,Karolina Haernvall,Sabine Zitzenbacher,Gernot Strohmeier,Oskar Hoff,Georg Steinkellner,Karl Gruber,Doris Ribitsch,Georg M. Guebitz +14 more
TL;DR: Interestingly, despite the lower activity on the soluble model substrate, the PA_PBM fusion enzyme was up to 4 times more active on the polymer when compared with the native enzyme, confirming the relevance of enzyme adsorption for efficient hydrolysis.