Showing papers by "Sanford A. Stass published in 2014"

Downregulation of miR-486-5p contributes to tumor progression and metastasis by targeting protumorigenic ARHGAP5 in lung cancer

Abstract: We have previously shown that miR-486-5p is one of the most downregulated micro RNAs in lung cancer The objective of the study was to investigate the role of miR-486-5p in the progression and metastasis of non-small-cell lung cancer (NSCLC) We evaluated miR-486-5p expression status on 76 frozen and 33 formalin-fixed paraffin-embedded tissues of NSCLC by quantitative reverse transcriptase PCR to determine its clinicopathologic significance We then performed function analysis of miR-486-5p to determine its potential roles on cancer cell migration and invasion in vitro and metastasis in vivo We also investigated the target genes of miR-486-5p in lung tumorigenesis miR-486-5p expression level was significantly lower in lung tumors compared with their corresponding normal tissues (P<00001), and associated with stage (P=00001) and lymph node metastasis of NSCLC (P=00019) Forced expression of miR-486-5p inhibited NSCLC cell migration and invasion in vitro and metastasis in mice by inhibiting cell proliferation Furthermore, ectopic expression of miR-486-5p in cancer cells reduced ARHGAP5 expression level, whereas miR-486-5p silencing increased its expression Luciferase assay demonstrated that miR-486-5p could directly bind to the 3'-untranslated region of ARHGAP5 The expression level of miR-486-5p was inversely correlated with that of ARHGAP5 in lung tumor tissues (P=00156) Reduced expression of ARHGAP5 considerably inhibited lung cancer cell migration and invasion, resembling that of miR-486-5p overexpression miR-486-5p may act as a tumor-suppressor contributing to the progression and metastasis of NSCLC by targeting ARHGAP5 miR-486-5p would provide potential diagnostic and therapeutic targets for the disease