http://ssu.ac.ir/cms/fileadmin/user_upload/Mtahghighat/tfood/ARTICLES/koliat/Natural_products_as_antimicrobial_agents.pdf

Natural products as antimicrobial agents

Bioactive peptides have been identified in a range of foods, including plant, milk and muscle, e.g., beef, chicken, pork and fish muscle proteins. Bioactive peptides from food proteins offer major potential for incorporation into functional foods and nutraceuticals. The aim of this paper is to present an outline of the bioactive peptides identified in the muscle protein of meat to date, with a focus on muscle protein from domestic animals and fish. The majority of research on bioactives from meat sources has focused on angiotensin-1-converting enzyme (ACE) inhibitory and antioxidant peptides.

/pdf/bioactive-peptides-from-muscle-sources-meat-and-fish-38rxoxs5hz.pdf

Bioactive peptides from muscle sources: meat and fish.

Bioactive peptides from meat muscle and by-products: generation, functionality and application as functional ingredients.

Antioxidant and antimicrobial peptidic hydrolysates from muscle protein sources and by-products

Biological activities and potential health benefits of bioactive peptides derived from marine organisms.

Purification and characterisation of antioxidative peptides from enzymatic hydrolysates of venison protein

This study was aimed at evaluating antibacterial and antioxidant activity of tannins extracted by several solvents from agricultural by-products, including green tea waste and acorn, chestnut, and persimmon hulls. Tannin content was the highest in acetone extracts from all types of hulls, but not green tea waste (p<0.05). Tannin extracts from green tea waste had higher antibacterial activity than those from acorn, chestnut, and persimmon hulls extracted with distilled water, ethanol, or acetone. With ethanol extraction, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity of green tea tannin extracts was significantly higher than that of tannins extracted from acorn, chestnut, and persimmon hulls (p<0.05). In particular, tannins extracted from green tea waste had a greater IC50 value for DPPH scavenging activity than did vitamin C. Antioxidant activity was also significantly higher in ethanol extracts from green tea waste than in distilled water and acetone extracts (p<0.05). Therefore, tannins extracted from green tea waste could be a natural source for substitutes to present antioxidants as well as antibiotics.

	 

	Key words: Acorn, antibacterial activity, antioxidant activity, chestnut, green tea, persimmon.

Antibacterial and antioxidant activities of tannins extracted from agricultural by-products

We applied enzymatic hydrolysis and tangential flow filtration (TFF) to purify a novel anticancer peptide from Mytilus coruscus (M. coruscus) and investigated its anticancer properties. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis. Pepsin hydrolysates, which showed clearly superior cytotoxic activity on prostate cancer cells, were further purified using a flow filtration system using a TFF and consecutive chromatographic methods. Finally, a novel anticancer peptide was obtained, and the sequence was identified as Ala-Phe-Asn-Ile-His- Asn-Arg-Asn-Leu-Leu. The peptide from M. coruscus effectively induced cell death on prostate, breast and lung cancer cells but not on normal liver cells. This is the first report of an anticancer peptide derived from the hydrolysates of M. coruscus.

Purification of a novel anticancer peptide from enzymatic hydrolysate of Mytilus coruscus.

A novel antioxidative peptide (APVPH I, antioxidative peptides from venison protein hydrolysates I) was purified from venison by enzymatic hydrolysis, column chromatography of DEAE-Sephacel and high performance liquid chromatography. The molecular weight of the purified peptide was found to be 9,853 Da and the amino acid sequences of the purified peptide was Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly. The purpose of this study was to evaluate the effects of APVPH I against H2O2-induced neuronal cells damage in PC-12 cells. Antioxidative enzyme levels in cultured neuronal cells were increased in the presence of the peptide. In addition, APVPH I inhibited productions of nitric oxide (NO), reactive oxygen species (ROS), malondialdehyde (MDA) and cell death against H2O2-induced neuronal cells damage in PC-12 cells. It was presumed to be APVPH I involved in regulating the apoptosis-related gene expression in the cell environment. Present results indicate APVPH I substantially contribute to antioxidative properties in neuronal cells.

Sang Ho Moon

Papers

Purification and characterisation of antioxidative peptides from enzymatic hydrolysates of venison protein

Antibacterial and antioxidant activities of tannins extracted from agricultural by-products

Purification of a novel anticancer peptide from enzymatic hydrolysate of Mytilus coruscus.

Neuroprotective effects of a novel peptide purified from venison protein.

Characterization of heparan sulfate from the unossified antler of Cervus elaphus