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Sarah A. Bade
Researcher at Iowa State University
Publications - 4
Citations - 104
Sarah A. Bade is an academic researcher from Iowa State University. The author has contributed to research in topics: Porcine reproductive and respiratory syndrome virus & Whole genome sequencing. The author has an hindex of 3, co-authored 4 publications receiving 74 citations. Previous affiliations of Sarah A. Bade include Chinese Academy of Sciences.
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Journal ArticleDOI
High-throughput whole genome sequencing of Porcine reproductive and respiratory syndrome virus from cell culture materials and clinical specimens using next-generation sequencing technology.
Jianqiang Zhang,Ying Zheng,Ying Zheng,Xiao-Qin Xia,Xiao-Qin Xia,Qi Chen,Qi Chen,Sarah A. Bade,Sarah A. Bade,Kyoung-Jin Yoon,Kyoung-Jin Yoon,Karen M. Harmon,Karen M. Harmon,Phillip C. Gauger,Phillip C. Gauger,Rodger Main,Rodger Main,Ganwu Li,Ganwu Li +18 more
TL;DR: The NGS approach described herein offers the prospect of high-throughput sequencing and could be adapted to routine workflows in veterinary diagnostic laboratories, although further improvement of sequencing outcomes from clinical specimens with higher Ct values remains to be investigated.
Journal ArticleDOI
Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus
Jianqiang Zhang,Charles Nfon,Chuan-Fu Tsai,Chien-Hsien Lee,Lindsay Fredericks,Qi Chen,Avanti Sinha,Sarah A. Bade,Karen M. Harmon,Pablo Piñeyro,Phillip C. Gauger,Yun-Long Tsai,Hwa-Tang Thomas Wang,Pei-Yu Alison Lee +13 more
TL;DR: The SVV rRT-PCR and RT-iiPCR developed in this study are very sensitive and specific and have comparable diagnostic performances for SVV RNA detection.
Journal ArticleDOI
Detection and genetic characterization of porcine pegivirus in pigs in the United States.
Chenghuai Yang,Leyi Wang,Huigang Shen,Ying Zheng,Sarah A. Bade,P. C. Gauger,Qi Chen,Jianqiang Zhang,Baoqing Guo,Kyoung-Jin Yoon,Karen M. Harmon,Rodger Main,Ganwu Li +12 more
TL;DR: Using next-generation sequencing on vesicular swab and serum from swine from the USA exhibiting lameness and vesicles, porcine pegivirus (PPgV) was first identified and genetically characterized in the United States.
Journal ArticleDOI
Effects of sample handling on the detection of porcine reproductive and respiratory syndrome virus in oral fluids by reverse-transcription real-time PCR.
Ashley C. Weiser,Korakrit Poonsuk,Sarah A. Bade,Phillip C. Gauger,Marisa L. Rotolo,Karen M. Harmon,Wendy Gonzalez,Chong Wang,Rodger Main,Jeffrey J. Zimmerman +9 more
TL;DR: The most significant factor affecting test outcome was thaw temperature (4°C or 25°C); samples thawed at 4°C had higher positivity rate (94% vs. 80%, p < 0.0001) and lower Ct (36.2 vs. 37.5, p <0.0001).