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Sarah D. Mahan
Researcher at Promega
Publications - 7
Citations - 306
Sarah D. Mahan is an academic researcher from Promega. The author has contributed to research in topics: Protein degradation & Ternary complex. The author has an hindex of 3, co-authored 7 publications receiving 150 citations.
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Journal ArticleDOI
Quantitative Live-Cell Kinetic Degradation and Mechanistic Profiling of PROTAC Mode of Action.
Kristin M. Riching,Sarah D. Mahan,Cesear Corona,Mark McDougall,James D Vasta,Matthew B. Robers,Marjeta Urh,Danette L. Daniels +7 more
TL;DR: An innovative, modular live-cell platform utilizing endogenous tagging technologies is presented and applied to monitoring PROTAC-mediated degradation of the bromodomain and extra-terminal family members, showing comprehensive real-time degradation and recovery profiles for each target.
Journal ArticleDOI
Trivalent PROTACs enhance protein degradation via combined avidity and cooperativity.
Satomi Imaide,Kristin M. Riching,Nikolai Makukhin,Vesna Vetma,Claire Whitworth,Scott J. Hughes,Nicole Trainor,Sarah D. Mahan,Nancy Murphy,Angus D. Cowan,Kwok-Ho Chan,Kwok-Ho Chan,Conner Craigon,Andrea Testa,Chiara Maniaci,Chiara Maniaci,Marjeta Urh,Danette L. Daniels,Alessio Ciulli +18 more
TL;DR: In this paper, a trivalent PROTAC consisting of a bivalent bromo and extra terminal (BET) inhibitor and an E3 ligand tethered via a branched linker was designed.
Journal ArticleDOI
Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications
Sweta Mishra,Capucine Van Rechem,Sangita Pal,Thomas L. Clarke,Damayanti Chakraborty,Sarah D. Mahan,Joshua C. Black,Sedona E. Murphy,Michael S. Lawrence,Michael S. Lawrence,Danette L. Daniels,Johnathan R. Whetstine +11 more
TL;DR: A collection of H 3K4-modifying chromatin regulators that function with H3K9 and H3k36 regulators to orchestrate TSSGs are uncovered and comprise an epigenetic addressing system for defining site-specific DNA rereplication and amplifications.
Journal ArticleDOI
Targeted Protein Degradation Phenotypic Studies Using HaloTag CRISPR/Cas9 Endogenous Tagging Coupled with HaloPROTAC3.
Elizabeth A. Caine,Sarah D. Mahan,Rebecca L. Johnson,Amanda N. Nieman,Ngan Lam,Curtis R. Warren,Kristin M. Riching,Marjeta Urh,Danette L. Daniels +8 more
TL;DR: This work presents a reversible and conditional live‐cell knockout strategy that is applicable to numerous proteins and regulates target loss at the protein, rather than the genomic, level through the use of HaloPROTAC3, which specifically degrades HaloTag fusion proteins via recruitment of the VHL E3 ligase component.
Journal ArticleDOI
High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds using HiBiT CRISPR Cell Lines.
TL;DR: This work presents a protocol and screening strategy based on the use of CRISPR/Cas9 endogenous tagging of target proteins with the 11 amino acid HiBiT tag which complements with high affinity to the LgBiT protein, to produce a luminescent protein.