I read this book the same weekend that the Packers took on the Rams, and the experience of the latter event, obviously, colored my judgment. Although I abhor anything that smacks of being a handbook (like, \"How to Earn a Merit Badge in Neurosurgery\") because too many volumes in biomedical science already evince a boyscout-like approach, I must confess that parts of this volume are fast, scholarly, and significant, with certain reservations. I like parts of this well-illustrated book because Dr. Sj6strand, without so stating, develops certain subjects on technique in relation to the acquisition of judgment and sophistication. And this is important! So, given that the author (like all of us) is somewhat deficient in some areas, and biased in others, the book is still valuable if the uninitiated reader swallows it in a general fashion, realizing full well that what will be required from the reader is a modulation to fit his vision, propreception, adaptation and response, and the kind of problem he is undertaking. A major deficiency of this book is revealed by comparison of its use of physics and of chemistry to provide understanding and background for the application of high resolution electron microscopy to problems in biology. Since the volume is keyed to high resolution electron microscopy, which is a sophisticated form of structural analysis, but really morphology in a modern guise, the physical and mechanical background of The instrument and its ancillary tools are simply and well presented. The potential use of chemical or cytochemical information as it relates to biological fine structure , however, is quite deficient. I wonder when even sophisticated morphol-ogists will consider fixation a reaction and not a technique; only then will the fundamentals become self-evident and predictable and this sine qua flon will become less mystical. Staining reactions (the most inadequate chapter) ought to be something more than a technique to selectively enhance contrast of morphological elements; it ought to give the structural addresses of some of the chemical residents of cell components. Is it pertinent that auto-radiography gets singled out for more complete coverage than other significant aspects of cytochemistry by a high resolution microscopist, when it has a built-in minimal error of 1,000 A in standard practice? I don't mean to blind-side (in strict football terminology) Dr. Sj6strand's efforts for what is \"routinely used in our laboratory\"; what is done is usually well done. It's just that …

Methods in Enzymology.

A drop hitting a solid surface can deposit, bounce, or splash. Splashing arises from the breakup of a fine liquid sheet that is ejected radially along the substrate. Bouncing and deposition depend crucially on the wetting properties of the substrate. In this review, we focus on recent experimental and theoretical studies, which aim at unraveling the underlying physics, characterized by the delicate interplay of not only liquid inertia, viscosity, and surface tension, but also the surrounding gas. The gas cushions the initial contact; it is entrapped in a central microbubble on the substrate; and it promotes the so-called corona splash, by lifting the lamella away from the solid. Particular attention is paid to the influence of surface roughness, natural or engineered to enhance repellency, relevant in many applications.

https://hal.archives-ouvertes.fr/hal-01398138/document

Drop Impact on a Solid Surface

Precise and effective control of droplet generation is critical for applications of droplet microfluidics ranging from materials synthesis to lab-on-a-chip systems. Methods for droplet generation can be either passive or active, where the former generates droplets without external actuation, and the latter makes use of additional energy input in promoting interfacial instabilities for droplet generation. A unified physical understanding of both passive and active droplet generation is beneficial for effectively developing new techniques meeting various demands arising from applications. Our review of passive approaches focuses on the characteristics and mechanisms of breakup modes of droplet generation occurring in microfluidic cross-flow, co-flow, flow-focusing, and step emulsification configurations. The review of active approaches covers the state-of-the-art techniques employing either external forces from electrical, magnetic and centrifugal fields or methods of modifying intrinsic properties of flows or fluids such as velocity, viscosity, interfacial tension, channel wettability, and fluid density, with a focus on their implementations and actuation mechanisms. Also included in this review is the contrast among different approaches of either passive or active nature.

Passive and active droplet generation with microfluidics: a review

The phase-shifting mask consists of a normal transmission mask that has been coated with a transparent layer patterned to ensure that the optical phases of nearest apertures are opposite. Destructive interference between waves from adjacent apertures cancels some diffraction effects and increases the spatial resolution with which such patterns can be projected. A simple theory predicts a near doubling of resolution for illumination with partial incoherence σ < 0.3, and substantial improvements in resolution for σ < 0.7. Initial results obtained with a phase-shifting mask patterned with typical device structures by electron-beam lithography and exposed using a Mann 4800 10× tool reveals a 40-percent increase in usuable resolution with some structures printed at a resolution of 1000 lines/mm. Phase-shifting mask structures can be used to facilitate proximity printing with larger gaps between mask and wafer. Theory indicates that the increase in resolution is accompanied by a minimal decrease in depth of focus. Thus the phase-shifting mask may be the most desirable device for enhancing optical lithography resolution in the VLSI/VHSIC era.

Improving resolution in photolithography with a phase-shifting mask

Droplet microfluidics offers exquisite control over the flows of multiple fluids in microscale, enabling fabrication of advanced microparticles with precisely tunable structures and compositions in a high throughput manner The combination of these remarkable features with proper materials and fabrication methods has enabled high efficiency, direct encapsulation of actives in microparticles whose features and functionalities can be well controlled These microparticles have great potential in a wide range of bio-related applications including drug delivery, cell-laden matrices, biosensors and even as artificial cells In this review, we briefly summarize the materials, fabrication methods, and microparticle structures produced with droplet microfluidics We also provide a comprehensive overview of their recent uses in biomedical applications Finally, we discuss the existing challenges and perspectives to promote the future development of these engineered microparticles

/pdf/microfluidic-fabrication-of-microparticles-for-biomedical-2tsrw0ufu5.pdf

Microfluidic fabrication of microparticles for biomedical applications

Dripping and jetting regimes in microfluidic multiphase flows have been investigated extensively, and this review summarizes the main observations and physical understandings in this field to date for three common device geometries: coaxial, flow-focusing and T-junction. The format of the presentation allows for simple and direct comparison of the different conditions for drop and jet formation, as well as the relative ease and utility of forming either drops or jets among the three geometries. The emphasis is on the use of drops and jets as templates for microparticle and microfiber syntheses, and a description is given of the more common methods of solidification and strategies for achieving complex multicomponent microparticles and microfibers.

https://iopscience.iop.org/article/10.1088/0022-3727/46/11/114002/pdf

Dripping and jetting in microfluidic multiphase flows applied to particle and fibre synthesis

When a liquid drop impacts a smooth, solid, dry surface, the drop forms a radially spreading lamella, which can lead to a splash. Previous studies have focused almost exclusively on impacts perpendicular to a surface; yet it is common for drops to impact on angled or moving surfaces. The asymmetry of such impacts leads to an azimuthal variation of the ejected rim, and under certain conditions only part of the rim breaks up to form droplets. We show that the tangential component of impact can act to enhance or suppress a splash. We develop a new model to predict when this type of splashing will occur. The model accounts for our observations of the effects of tangential velocity and agrees well with previous experimental data.

/pdf/inclined-to-splash-triggering-and-inhibiting-a-splash-with-4fun51gpwd.pdf

Inclined to splash: triggering and inhibiting a splash with tangential velocity

Arsenic contamination of drinking water is a major global problem, with contamination in Bangladesh deemed most serious. Although the current World Health Organisation (WHO) maximum contamination limit (MCL) for arsenic in drinking water is 10 μg L−1, due to practical and economic constraints, the standard limit in Bangladesh and many other developing nations is 50 μg L−1. We propose that an ideal arsenic sensor, designed for routine monitoring, will have five essential qualities: sensitivity and selectivity for arsenic; speed and reliability; portability and robustness; reduced health and environmental risks; and affordability and ease of use for local technicians. It is our opinion that many of these characteristics can be accentuated by microfluidic systems. We describe candidate colorimetric, electrochemical, biological, electrophoretic, surface-sensing, and spectroscopic methods for arsenic detection; and comment on their potential for portable microfluidic adaptation. We also describe existing developments in the literature towards the ultimate creation of microfluidic total analysis systems (μTASs) for arsenic detection. The fundamental purpose of this review is to highlight the need for better portable arsenic contamination detection, and describe how microfluidic technology may be developed to address this need.

Detection of trace arsenic in drinking water: challenges and opportunities for microfluidics

We present a simple microfluidic system that generates water-in-water, aqueous two phase system (ATPS) droplets, by passive flow focusing. ATPS droplet formation is achieved by applying weak hydrostatic pressures, with liquid-filled pipette tips as fluid columns at the inlets, to introduce low speed flows to the flow focusing junction. To control the size of the droplets, we systematically vary the interfacial tension and viscosity of the ATPS fluids and adjust the fluid column height at the fluid inlets. The size of the droplets scales with a power law of the ratio of viscous stresses in the two ATPS phases. Overall, we find a drop size coefficient of variation (CV; i.e., polydispersity) of about 10%. We also find that when drops form very close to the flow focusing junction, the drops have a CV of less than 1%. Our droplet generation method is easily scalable: we demonstrate a parallel system that generates droplets simultaneously and improves the droplet production rate by up to one order of magnitude....

Water-in-Water Droplets by Passive Microfluidic Flow Focusing.

We present a technique that generates droplets using ultralow interfacial tension aqueous two-phase systems (ATPS). Our method combines a classical microfluidic flow focusing geometry with precisely controlled pulsating inlet pressure, to form monodisperse ATPS droplets. The dextran (DEX) disperse phase enters through the central inlet with variable on–off pressure cycles controlled by a pneumatic solenoid valve. The continuous phase polyethylene glycol (PEG) solution enters the flow focusing junction through the cross channels at a fixed flow rate. The on–off cycles of the applied pressure, combined with the fixed flow rate cross flow, make it possible for the ATPS jet to break up into droplets. We observe different droplet formation regimes with changes in the applied pressure magnitude and timing, and the continuous phase flow rate. We also develop a scaling model to predict the size of the generated droplets, and the experimental results show a good quantitative agreement with our scaling model. Additionally, we demonstrate the potential for scaling-up of the droplet production rate, with a simultaneous two-droplet generating geometry. We anticipate that this simple and precise approach to making ATPS droplets will find utility in biological applications where the all-biocompatibility of ATPS is desirable.

Scott S. H. Tsai

Papers

Dripping and jetting in microfluidic multiphase flows applied to particle and fibre synthesis

Inclined to splash: triggering and inhibiting a splash with tangential velocity

Detection of trace arsenic in drinking water: challenges and opportunities for microfluidics

Water-in-Water Droplets by Passive Microfluidic Flow Focusing.

Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures