S
Severine Gharbi
Researcher at Spanish National Research Council
Publications - 23
Citations - 1544
Severine Gharbi is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Immunological synapse & Diacylglycerol kinase. The author has an hindex of 16, co-authored 21 publications receiving 1466 citations. Previous affiliations of Severine Gharbi include Ludwig Institute for Cancer Research.
Papers
More filters
Journal ArticleDOI
Exploring the specificity of the PI3K family inhibitor LY294002.
Severine Gharbi,Marketa Zvelebil,Stephen J. Shuttleworth,Tim Hancox,Nahid Saghir,John F. Timms,John F. Timms,Michael D. Waterfield +7 more
TL;DR: The present study reveals that LY294002 not only binds to class I PI3Ks and otherPI3K-related kinases, but also to novel targets seemingly unrelated to the PI3k family.
Journal ArticleDOI
Evaluation of two-dimensional differential gel electrophoresis for proteomic expression analysis of a model breast cancer cell system.
Severine Gharbi,Piers R. J. Gaffney,Alice Yang,Marketa Zvelebil,Rainer Cramer,Michael D. Waterfield,John F. Timms +6 more
TL;DR: SyproRuby staining was shown to be a highly sensitive and 2D difference gel electrophoresis-compatible method for post-electrophoretic visualization of proteins, which could then be picked and identified by matrix-assisted laser-desorption ionization mass spectroscopy.
Journal ArticleDOI
Class IA phosphoinositide 3-kinases are obligate p85-p110 heterodimers.
TL;DR: It is demonstrated that the p85 and p110 subunits are present in equimolar amounts in mammalian cell lines and tissues and insights into the nonredundant functions of the different class IA PI3K isoforms are provided.
Journal ArticleDOI
Proteomic analysis of redox- and ErbB2-dependent changes in mammary luminal epithelial cells using cysteine- and lysine-labelling two-dimensional difference gel electrophoresis.
Hong-Lin Chan,Severine Gharbi,Piers R. J. Gaffney,Rainer Cramer,Michael D. Waterfield,John F. Timms +5 more
TL;DR: This study identifies differentially modified proteins involved in cellular redox regulation, protein folding, proliferative suppression, glycolysis and cytoskeletal organisation, revealing the complexity of the response to oxidative stress and the impact that overexpression of ErbB2 has on this response.
Journal ArticleDOI
Redundant and specialized roles for diacylglycerol kinases α and ζ in the control of T cell functions
TL;DR: This Review highlights how the study of DGKs and the signals that they regulate is important because it will help scientists learn how to manipulate T cell responses for the treatment of diseases.