THE DESIGN AND ANALYSIS OF EXPERIMENTS. By Oscar Kempthorne. New York, John Wiley and Sons, Inc., 1952. 631 pp. $8.50. This book by a teacher of statistics (as well as a consultant for \"experimenters\") is a comprehensive study of the philosophical background for the statistical design of experiment. It is necessary to have some facility with algebraic notation and manipulation to be able to use the volume intelligently. The problems are presented from the theoretical point of view, without such practical examples as would be helpful for those not acquainted with mathematics. The mathematical justification for the techniques is given. As a somewhat advanced treatment of the design and analysis of experiments, this volume will be interesting and helpful for many who approach statistics theoretically as well as practically. With emphasis on the \"why,\" and with description given broadly, the author relates the subject matter to the general theory of statistics and to the general problem of experimental inference. MARGARET J. ROBERTSON

The Design and Analysis of Experiments

Osteochondral defects are prone to induce osteoarthritic degenerative changes. Many tissue-engineering approaches that aim to generate osteochondral implants suffer from poor tissue formation and compromised integration. This illustrates the need for further improvement of heterogeneous tissue constructs. Engineering of these structures is expected to profit from strategies addressing the complexity of tissue organization and the simultaneous use of multiple cell types. Moreover, this enables the investigation of the effects of three-dimensional (3D) organization and architecture on tissue function. In the present study, we characterize the use of a 3D fiber deposition (3DF) technique for the fabrication of cell-laden, heterogeneous hydrogel constructs for potential use as osteochondral grafts. Changing fiber spacing or angle of fiber deposition yielded scaffolds of varying porosity and elastic modulus. We encapsulated and printed fluorescently labeled human chondrocytes and osteogenic progenitors in alginate hydrogel yielding scaffolds of 1×2 cm with different parts for both cell types. Cell viability remained high throughout the printing process, and cells remained in their compartment of the printed scaffold for the whole culture period. Moreover, distinctive tissue formation was observed, both in vitro after 3 weeks and in vivo (6 weeks subcutaneously in immunodeficient mice), at different locations within one construct. These results demonstrate the possibility of manufacturing viable centimeter-scaled structured tissues by the 3DF technique, which could potentially be used for the repair of osteochondral defects.

Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds

A Review of 3D Printing Technologies for Soft Polymer Materials

Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering

In situ bioprinting - Bioprinting from benchside to bedside?

Bioprinting functional tissues

Expediting Build Time, Material, and Cost Estimation for Material Extrusion Processes to Enable Mobile Applications

Research suggests that topology and build parameters in Fused Filament Fabrication (FFF) play a vital role in determining mechanical properties of parts produced by this technique. In particular, the use of 2D layers printed parallel to the build surface produces high anisotropy in parts making them the weakest when loaded perpendicular to the layer interfaces. We investigate a novel approach that uses non-planar 3D layer shapes Active Z printing, to improve mechanical strength through alignment of localized stress tensors parallel to the deposition paths. Sinusoidal layer shapes are used with varying amplitude, frequency, and orientation. Design of experiments is performed to correlate effect of varying shape and orientation of sinusoidal layer shapes on flexural strength of parts. Based on this, the results are used to decide parameters to be studied further and characterize their effect on the strength of parts.

Shantanab Dinda

Papers

Bioprinting functional tissues

Expediting Build Time, Material, and Cost Estimation for Material Extrusion Processes to Enable Mobile Applications

Active - Z Printing: A New Approach to Increasing 3D Printed Part Strength

Education Readiness Levels (ERLs): A Scale for Assessing Educational Coursework and Training Modules

Redesign of the femoral stem for a total hip arthroplasty for additive manufacturing