Soil Chemical Analysis

The aim of this review is to assess the mode of action and role of antioxidants as protection from heavy metal stress in roots, mycorrhizal fungi and mycorrhizae. Based on their chemical and physical properties three different molecular mechanisms of heavy metal toxicity can be distinguished: (a) production of reactive oxygen species by autoxidation and Fenton reaction; this reaction is typical for transition metals such as iron or copper, (b) blocking of essential functional groups in biomolecules, this reaction has mainly been reported for non-redox-reactive heavy metals such as cadmium and mercury, (c) displacement of essential metal ions from biomolecules; the latter reaction occurs with different kinds of heavy metals. Transition metals cause oxidative injury in plant tissue, but a literature survey did not provide evidence that this stress could be alleviated by increased levels of antioxidative systems. The reason may be that transition metals initiate hydroxyl radical production, which can not be controlled by antioxidants. Exposure of plants to non-redox reactive metals also resulted in oxidative stress as indicated by lipid peroxidation, H(2)O(2) accumulation, and an oxidative burst. Cadmium and some other metals caused a transient depletion of GSH and an inhibition of antioxidative enzymes, especially of glutathione reductase. Assessment of antioxidative capacities by metabolic modelling suggested that the reported diminution of antioxidants was sufficient to cause H(2)O(2) accumulation. The depletion of GSH is apparently a critical step in cadmium sensitivity since plants with improved capacities for GSH synthesis displayed higher Cd tolerance. Available data suggest that cadmium, when not detoxified rapidly enough, may trigger, via the disturbance of the redox control of the cell, a sequence of reactions leading to growth inhibition, stimulation of secondary metabolism, lignification, and finally cell death. This view is in contrast to the idea that cadmium results in unspecific necrosis. Plants in certain mycorrhizal associations are less sensitive to cadmium stress than non-mycorrhizal plants. Data about antioxidative systems in mycorrhizal fungi in pure culture and in symbiosis are scarce. The present results indicate that mycorrhization stimulated the phenolic defence system in the Paxillus-Pinus mycorrhizal symbiosis. Cadmium-induced changes in mycorrhizal roots were absent or smaller than those in non-mycorrhizal roots. These observations suggest that although changes in rhizospheric conditions were perceived by the root part of the symbiosis, the typical Cd-induced stress responses of phenolics were buffered. It is not known whether mycorrhization protected roots from Cd-induced injury by preventing access of cadmium to sensitive extra- or intracellular sites, or by excreted or intrinsic metal-chelators, or by other defence systems. It is possible that mycorrhizal fungi provide protection via GSH since higher concentrations of this thiol were found in pure cultures of the fungi than in bare roots. The development of stress-tolerant plant-mycorrhizal associations may be a promising new strategy for phytoremediation and soil amelioration measures.

Plant responses to abiotic stresses: heavy metal‐induced oxidative stress and protection by mycorrhization

Reactive oxygen species (ROS) were initially recognized as toxic by-products of aerobic metabolism. In recent years, it has become apparent that ROS plays an important signaling role in plants, controlling processes such as growth, development and especially response to biotic and abiotic environmental stimuli. The major members of the ROS family include free radicals like O2● −, OH● and non-radicals like H2O2 and 1O2. The ROS production in plants is mainly localized in the chloroplast, mitochondria and peroxisomes. There are secondary sites as well like the endoplasmic reticulum, cell membrane, cell wall and the apoplast. The role of the ROS family is that of a double edged sword; while they act as secondary messengers in various key physiological phenomena, they also induce oxidative damages under several environmental stress conditions like salinity, drought, cold, heavy metals, UV irradiation etc., when the delicate balance between ROS production and elimination, necessary for normal cellular homeostasis, is disturbed. The cellular damages are manifested in the form of degradation of biomolecules like pigments, proteins, lipids, carbohydrates and DNA, which ultimately amalgamate in plant cellular death. To ensure survival, plants have developed efficient antioxidant machinery having two arms, (i) enzymatic components like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR); (ii) non-enzymatic antioxidants like ascorbic acid (AA), reduced glutathione (GSH), α-tocopherol, carotenoids, flavonoids and the osmolyte proline. These two components work hand in hand to scavenge ROS. In this review, we emphasize on the different types of ROS, their cellular production sites, their targets, and their scavenging mechanism mediated by both the branches of the antioxidant systems, highlighting the potential role of antioxidant

Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants

B6nassy, C., 1955. R6marques sur deux Aphelinid6s: Aphelinus mytilaspidis Le Baron et Aphytis proclia Walker. Annls l~piphyt. 6: 11-17. Lord, F. T. & MacPhee, A. W., 1953. The influence of spray programs on the fauna of apple orchards in Nova Scotia II. Oyster shell scale. Can. Ent. 79: 196-209. Pickett, A. D., 1946. A progress report on long term spray programs. Rep. Nova Scotia Fruit Grow. Ass. 83 : 27-31. Pickett, A. D., 1967. The influence of spray programs on the fauna of apple orchards in Nova Scotia XIV. Can. Ent. 97: 816-821. Tothill, J. D., 1918. The predacious mite Hemisarcoptes malus Shimer and its relation to the natural control of the oyster shell scale Lepidosaphes ulmi L. Agric. Gaz. Can. 5 : 234-239.

http://link.springer.com/content/pdf/10.1007/BF01976688.pdf

Soil enzymes: Kuprevich, V. F. & Shcherbakova, T. A.: Translated from the Russian edition (1966), published by the Indian National Scientific Documentation Centre, New Delhi 1971. 392 pp., offset printing from type script, paper cover. Obtainable from U.S. Department of Commerce, National Technical Information Service, Springfield, Va. 22151

Plants exposed to heavy metals accumulate an array of metabolites, some to high millimolar concentrations. This review deals with N-containing metabolites frequently preferentially synthesized under heavy metal stress such as Cd, Cu, Ni, and Zn. Special focus is given to proline, but certain other amino acids and oligopeptides, as well as betaine, polyamines, and nicotianamine are also addressed. Particularly for proline a large body of data suggests significant beneficial functions under metal stress. In general, the molecules have three major functions, namely metal binding, antioxidant defence, and signalling. Strong correlative and mechanistic experimental evidence, including work with transgenic plants and algae, has been provided that indicates the involvement of metal-induced proline in metal stress defence. Histidine, other amino acids and particularly phytochelatins and glutathione play a role in metal binding, while polyamines function as signalling molecules and antioxidants. Their accumulation needs to be considered as active response and not as consequence of metabolic dys-regulation.

The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress

Global Climate Change and Crop Responses: Uncertainties Associated With the Current Methodologies - S.V. Krupa and J.V. Groth The Effects of Climate Change on the Behavior of Woody Perennials - C.J. Atkinson CO2 Enrichment of the Atmosphere and Water Economy of Plants - J. Heath and T.A. Mansfield Plant Responses to elevated CO2: A Perspective From Natural CO2 Springs - M. Bandiani, A. Raschi, A.R. Paolacci, and F. Miglietta UV Effects on Plants - M. Tevini Field Studies on Impacts of Air Pollution on Agricultural Crops - J.N.B. Bell and F.M. Marshall Air Pollution and Vegetation Damage in South America: State of Knowledge and Perspectives - A. Klumpp, M. Domingos, and M.L. Pignata Effects of Air Pollution on Plant Diversity - M. Agrawal and S.B. Agrawal Effects of Tropospheric Ozone on Woody Plants - K. Bortier, R. Ceulemans, and L. De Temmerman Extracellular Antioxidants: A Protective Screen Against Ozone?- T. Lyons, M.Plochl, E. Turcsinyi and J. Barnes Early Detection Mechanisms of Tolerance, and Amelioration of Ozone Stress in Crop Plants - E.H. Lee Defense Strategies against Ozone in Trees: The Role of Nutrition - A.Polle, R. Matyssek, M.S. Gunthardt-Georg, and S. Maurer Use of Protective Chemicals to Assess the Effects of Ambient Ozone on Plants - W.J. Manning Sources, Atmospheric Transport and Sinks of Tropospheric Nitrous and Nitric Acids - R. Zimmerling and U. Dammen Effects of Sulfur Dioxide and Acid Deposition on Chinese Crops - C. Hongfa, J. Shu, Y. Shen, Y. Gao, and L. Zhang The Use of Calibrated Passive Monitors to Assess Crop Loss Due to Ozone in Rural Locations - V.C. Runeckles and P.A. Bowen Wild Plant and Crop Plant Species for "in situ" Microspore Analysis of a Polluted Environment - G. Murin and K. Mieieta Phytomonitoring in Industrial Areas - S.B. Chaphekar Statistical Baseline Values for Chemical Elements in the Lichen Hypogymnia physodes - J.P. Bennet Monitoring Air Pollutant Deposition in the Arctic with a Lichen by Means of Microscopy and Energy-Dispersive X-Ray Microanalysis: A Case Study - R.Crang Phytochelation and Metal Tolerance -R.K. Mehra and R.D. Tripathi

Environmental pollution and plant responses

Changes in oxidative stress defense system in wheat (Triticum aestivum L.) and mung bean (Vigna radiata L.) cultivars grown with and without mineral nutrients and irradiated by supplemental ultraviolet-B

Heavy metals released into the water bodies and on land surfaces by industries are highly toxic and carcinogenic in nature. These heavy metals create serious threats to all the flora and fauna due to their bioaccumulatory and biomagnifying nature at various levels of food chain. Existing conventional technologies for heavy metal removal are witnessing a downfall due to high operational cost and generation of huge quantity of chemical sludge. Adsorption by various adsorbents appears to be a potential alternative of conventional technologies. Its low cost, high efficiency, and possibility of adsorbent regeneration for reuse and recovery of metal ions for various purposes have allured the scientists to work on this technique. The present review compiles the exhaustive information available on the utilization of bacteria, algae, fungi, endophytes, aquatic plants, and agrowastes as source of adsorbent in adsorption process for removal of heavy metals from aquatic medium. During the last few years, a lot of work has been conducted on development of adsorbents after modification with various chemical and physical techniques. Adsorption of heavy metal ions is a complex process affected by operating conditions. As evident from the literature, Langmuir and Freundlich are the most widely used isotherm models, while pseudo first and second order are popularly studied kinetic models. Further, more researches are required in continuous column system and its practical application in wastewater treatment.

A review on progress of heavy metal removal using adsorbents of microbial and plant origin.

Ultraviolet-B (UV-B) radiation has, for many decades now, been widely studied with respect to its consequences on plant and animal health. Though according to NASA, the ozone hole is on its way to recovery, it will still be a considerable time before UV-B levels reach pre-industrial limits. Thus, for the present, excessive UV-B reaching the Earth is a cause for concern, and UV-B related human ailments are on the rise. Plants produce various secondary metabolites as one of the defense strategies under UV-B. They provide photoprotection via their UV-B screening effects and by quenching the reactive oxygen- and nitrogen species produced under UV-B influence. These properties of plant secondary metabolites (PSMs) are being increasingly recognized and made use of in sunscreens and cosmetics, and pharma- and nutraceuticals are gradually becoming a part of the regular diet. Secondary metabolites derived from medicinal plants (alkaloids, terpenoids, and phenolics) are a source of pharmaceuticals, nutraceuticals, as well as more rigorously tested and regulated drugs. These metabolites have been implicated in providing protection not only to plants under the influence of UV-B, but also to animals/animal cell lines, when the innate defenses in the latter are not adequate under UV-B-induced damage. The present review focuses on the defense potential of secondary metabolites derived from medicinal plants in both plants and animals. In plants, the concentrations of the alkaloids, terpenes/terpenoids, and phenolics have been discussed under UV-B irradiation as well as the fate of the genes and enzymes involved in their biosynthetic pathways. Their role in providing protection to animal models subjected to UV-B has been subsequently elucidated. Finally, we discuss the possible futuristic scenarios and implications for plant, animal, and human health pertaining to the defense potential of these secondary metabolites under UV-B radiation-mediated damages.

Defense potential of secondary metabolites in medicinal plants under UV-B stress

http://www.indiaenvironmentportal.org.in/files/Assessment%20of%20yield%20losses%20in%20tropical%20wheat.pdf

Shashi Bhushan Agrawal

Papers

Environmental pollution and plant responses

Changes in oxidative stress defense system in wheat (Triticum aestivum L.) and mung bean (Vigna radiata L.) cultivars grown with and without mineral nutrients and irradiated by supplemental ultraviolet-B

A review on progress of heavy metal removal using adsorbents of microbial and plant origin.

Defense potential of secondary metabolites in medicinal plants under UV-B stress

Assessment of yield losses in tropical wheat using open top chambers