S
Shelley Grimes
Researcher at University of Minnesota
Publications - 47
Citations - 4714
Shelley Grimes is an academic researcher from University of Minnesota. The author has contributed to research in topics: Prohead & DNA. The author has an hindex of 28, co-authored 46 publications receiving 4457 citations.
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Journal ArticleDOI
The bacteriophage φ29 portal motor can package DNA against a large internal force
Douglas E. Smith,Sander J. Tans,Steven B. Smith,Shelley Grimes,Dwight L. Anderson,Carlos Bustamante +5 more
TL;DR: The force–velocity relationship of the motor is established and it is found that the rate-limiting step of the machine's cycle is force dependent even at low loads, suggesting that this force may be available for initiating the ejection of the DNA from the capsid during infection.
Journal ArticleDOI
Structure of the bacteriophage φ29 DNA packaging motor
Alan A. Simpson,Yizhi Jane Tao,Yizhi Jane Tao,Petr G. Leiman,Mohammed O. Badasso,Yongning He,Paul J. Jardine,Norman H. Olson,Marc C. Morais,Shelley Grimes,Dwight L. Anderson,Timothy S. Baker,Michael G. Rossmann +12 more
TL;DR: In this article, the structure of the head-tail connector of the Bacillus subtilis bacteriophage phi29 was determined by means of X-ray crystallography, and the connector was fitted into the electron densities of the prohead and of the partially packaged prohead.
Journal ArticleDOI
Mechanism of force generation of a viral DNA packaging motor.
Yann R. Chemla,K. Aathavan,Jens Michaelis,Shelley Grimes,Paul J. Jardine,Dwight L. Anderson,Carlos Bustamante +6 more
TL;DR: The kinetic parameters of the packaging motor and their dependence on external load are determined to show that DNA translocation does not occur during ATP binding but is likely triggered by phosphate release, and a minimal mechanochemical cycle of this DNA-translocating ATPase is proposed.
Journal ArticleDOI
Intersubunit coordination in a homomeric ring ATPase
Jeffrey R. Moffitt,Yann R. Chemla,K. Aathavan,Shelley Grimes,Paul J. Jardine,Dwight L. Anderson,Carlos Bustamante +6 more
TL;DR: The direct observation of the intersubunit coordination and step size of a ring ATPase, the double-stranded-DNA packaging motor in the bacteriophage ϕ29, indicates that the hydrolysis cycles of the individual subunits are highly coordinated by means of a mechanism novel for ring ATPases.
motor can package DNA against a large internal force
Douglas E. Smith,Sander J. Tans,Steven B. Smith,Shelley Grimes,Dwight L. Anderson,Carlos Bustamante +5 more
TL;DR: The force-velocity relationship of the motor is established and it is found that the rate-limiting step of the machine's cycle is force dependent even at low loads, suggesting that this force may be available for initiating the ejection of the DNA from the capsid during infection.