Shiran Bar

TL;DR: It is found that the TP53 mutant allelic fraction increased with passage number under standard culture conditions, suggesting that the P53 mutations confer selective advantage, and it is suggested that careful genetic characterization of hPS cells and their differentiated derivatives be carried out before clinical use.

TL;DR: In this article, the authors highlight frequent epigenetic aberrations found in human pluripotent stem cells, including alterations in DNA methylation patterns, parental imprinting, and X chromosome inactivation.

TL;DR: It is shown that reprogrammed hPSCs acquire higher levels of LOI compared with embryonic stem cells and that LOI can pre-exist in their somatic cells of origin, and that those controlled paternally are more prone to disruption.

TL;DR: While the majority of iPSC lines maintain an inactive X chromosome, ESC lines tend to silence the expression of XIST and upregulate distal chromosomal regions, which suggest differences in XCI status between most published samples of embryonic stem cells and induced PSCs.

TL;DR: This study provides a global analysis of tissue-specific imprinting in humans and suggests that alternative promoters are central in the regulation of imprinted genes.