Sophia V. Kyriacou

TL;DR: Assessment of real-time change of membrane permeability and pore sizes of P. aeruginosa using the intrinsic color index (surface plasmon resonance spectra) of silver (Ag) nanoparticles as the nanometer size index probes shows that Ag nanoparticles with sizes ranging up to 80 nm are accumulated in living microbial cells, demonstrating that these Ag nanoparticle transport through the inner and outer membrane of the cells.

TL;DR: It is demonstrated that the number of Ag nanoparticles accumulated in cells increases as the aztreonam (AZT) concentration increases and as incubation time increases, showing that AZT induces the sized transformation of membrane permeability and the disruption of the cell wall.

TL;DR: An extraordinary heterogeneity of size and dynamics of membrane transport mechanisms was observed, demonstrating real-time transformation of membrane permeability and an active extrusion system.

TL;DR: Time courses of fluorescence intensity of these three strains in ethidium bromide (EtBr) showed that accumulation kinetics and extrusion machinery were highly dependent upon pump substrate concentration and an inductive mechanism in the development of substrate resistance in P. aeruginosa was suggested.

TL;DR: This research constitutes the first direct imaging of resistance mechanism of live bacterial cells at single cell resolution and opens up the new possibility of advancing the understanding of bacteria resistance mechanism.