Sridhar Gopishetty

TL;DR: The source of NADPH-dependent cytosolic 3β-hydroxysteroid dehydrogenase (3β-HSD) activity is unknown to date as mentioned in this paper, but it has been shown that AKR1C catalyzes the reduction of DHT into both 3α- and 3βDiol (established by 1H NMR spectroscopy).

TL;DR: Crystallographic and molecular modeling studies showed that the carboxylic acid of the inhibitor ligand was tethered by the catalytic Tyr55-OH2+ and explained why A-ring substituted N-phenylanthranilates inhibited only AKR1C enzymes, and these compounds can be used to dissect the role of the AKR 1C isozymes in neoplastic diseases and may have cancer chemopreventive roles independent of COX inhibition.

TL;DR: To the authors' knowledge, this is the first report of theophylline N demethylation and coexpression of distinct pathways for caffeine and theophyLLine degradation in bacteria.

TL;DR: Spectrophotometric assays showed that NADPH caused PAH o-quinones to enter futile redox cycles, which result in the depletion of excess cofactor, and that the immediate oxidant was not hydroxyl radical or Cu(I)OOH and that it is more likely (1)O(2), which can produce a 4,8-endoperoxide-dGuo intermediate.

TL;DR: Various biotechnological applications of these genes responsible for bacterial caffeine degradation, including bio‐decaffeination, remediation of caffeine‐contaminated environments, production of chemical and fuels and development of diagnostic tests have also been demonstrated.