Stanton B. Gelvin

TL;DR: This second edition of the Plant Molecular Biology Manual contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, and nuclear protein targeting, not previously described in the first edition.

TL;DR: The construction of new helper Ti plasmids for Agrobacterium-mediated plant transformation using T-DNA regions deleted using site-directed mutagenesis to yield replicons carrying thevir genes that will complement binary vectorsin trans.

TL;DR: Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.

TL;DR: What is currently known about the functions of virulence and plant proteins in several aspects of the Agrobacterium transformation process is reviewed.

TL;DR: The state of plant transformation is reviewed and innovations needed to enable genome editing in crops are pointed to, including a potential game-changer in crop genetics when plant transformation systems are optimized.