Elizabeth E. Hood

TL;DR: The construction of new helper Ti plasmids for Agrobacterium-mediated plant transformation using T-DNA regions deleted using site-directed mutagenesis to yield replicons carrying thevir genes that will complement binary vectorsin trans.

TL;DR: It is suggested that the hypervirulence of Agrobacterium tumefaciens A281 is due to non-T-DNA sequences on the Ti plasmid.

TL;DR: In this article, a transformant showing high-level expression of avidin was selected, and the protein was purified to greater than 90% purity by affinity chromatography after extraction from ground mature maize seed, showing that the N-terminal amino acid sequence and biotin binding characteristics are identical to the native protein with near identical molecular weight and glycosylation.

TL;DR: This work expresses the B-subunit of Escherichia coli heat-labile enterotoxin and the spike protein of swine transmissible gastroenteritis virus at high levels in corn, and demonstrates that these antigens delivered in the seed elicit protective immune responses.

TL;DR: Commercial‐level production of trypsin in transgenic maize (Zea mays) and its physical and functional characterization shows that this protease, the first enzyme to be produced on a large‐scale using transgenic plant technology, is functionally equivalent to native bovine pancreatictrypsin.