S
Stefan Löfås
Researcher at Uppsala University
Publications - 18
Citations - 4298
Stefan Löfås is an academic researcher from Uppsala University. The author has contributed to research in topics: Surface plasmon resonance & Biosensor. The author has an hindex of 14, co-authored 18 publications receiving 4246 citations.
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Journal ArticleDOI
Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors
TL;DR: The biospecific interaction of the immobilized ligand with its biological counterpart is illustrated by the effects on the interaction of immunoglobulins with immobilized Staphylococcus aureus protein A for various amounts of protein A.
Journal Article
Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology.
Jönsson U,Lars G. Fägerstam,B Ivarsson,B Johnsson,Robert Karlsson,K Lundh,Stefan Löfås,Björn Persson,Håkan Roos,I Rönnberg +9 more
TL;DR: The development and application of a biosensor-based technology that employs surface plasmon resonance for label-free studies of molecular interactions in real time and the ability to monitor multi-molecular complexes as they form are reported on.
Journal ArticleDOI
A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands
Stefan Löfås,Bo Johnsson +1 more
TL;DR: Gold surfaces intended for surface plasmon resonance sensors have been derivatized with a flexible matrix composed of carboxymethylated dextran to provide fast and efficient coupling of proteins and other ligands at low concentrations using the principles of ionic pre-concentration.
Journal ArticleDOI
Comparison of methods for immobilization to carboxymethyl dextran sensor surfaces by analysis of the specific activity of monoclonal antibodies
TL;DR: Studies show that, with the flexible carboxymethyl dextran matrix used here, the immobilization methods give rise to only minor differences in specific activity, and a ‘site directed’ immobilization strategy for monoclonal antibodies has no advantage.
Journal ArticleDOI
Methods for site controlled coupling to carboxymethyldextran surfaces in surface plasmon resonance sensors
Stefan Löfås,Bo Johnsson,Åsa Edström,Anna Hansson,Grabrielle Lindquist,Rose-Marie Müller Hillgren,Lena Stigh +6 more
TL;DR: Methods for in-situ ligand immobilization to carboxymethylated dextran coated gold surfaces for use in surface plasmon resonance (SPR) based biosensor devices were developed and found to work efficiently in combination with conditions suitable for electrostatic preconcentration of ligands to be coupled.