S
Stephen O'Gorman
Researcher at Salk Institute for Biological Studies
Publications - 19
Citations - 3264
Stephen O'Gorman is an academic researcher from Salk Institute for Biological Studies. The author has contributed to research in topics: Recombinase & Gene. The author has an hindex of 14, co-authored 19 publications receiving 3181 citations. Previous affiliations of Stephen O'Gorman include Stanford University & University of California, San Francisco.
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Recombinase-mediated gene activation and site-specific integration in mammalian cells.
TL;DR: The results suggest that FLP could be used to mosaically activate or inactivate transgenes for analysis of vertebrate development, and to efficiently integrate transfected DNA at predetermined chromosomal locations.
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Protamine-Cre recombinase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells
TL;DR: It is shown that transgenes comprised of the mouse protamine 1 promoter and the Cre recombinase coding sequence mediate the efficient recombination of a Cre target transgene in the male germ line, but not in other tissues.
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Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3'-kinase is essential for male fertility.
TL;DR: The Cre-loxP system is employed to mutate the codon for Tyr719, the PI 3′-kinase binding site in Kit/SCF-R, to Phe in the genome of mice by homologous recombination, providing the first in vivo model for male sterility caused by a discrete signalling pathway defect affecting early germ cells.
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Requirement for Pbx1 in skeletal patterning and programming chondrocyte proliferation and differentiation
Licia Selleri,Michael J. Depew,Yakop Jacobs,Sumit K. Chanda,Kwok Ying Tsang,Kathryn S.E. Cheah,John L.R. Rubenstein,Stephen O'Gorman,Michael L. Cleary +8 more
TL;DR: A role for Pbx1 is demonstrated in multiple developmental programs and a novel function in co-ordinating the extent and/or timing of proliferation with terminal differentiation is revealed, which impacts on the rate of endochondral ossification and bone formation and suggests a mechanistic basis for most of the observed skeletal malformations.
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A central role for chromosome breakage in gene amplification, deletion formation, and amplicon integration.
TL;DR: A CHO cell line with a single copy of the DHFR locus on chromosome Z2 was used to analyze the structure of the amplification target and products subsequent to the initial amplification event as mentioned in this paper.