S
Stewart A. Noble
Researcher at Research Triangle Park
Publications - 8
Citations - 1296
Stewart A. Noble is an academic researcher from Research Triangle Park. The author has contributed to research in topics: Nucleic acid & DNA. The author has an hindex of 6, co-authored 8 publications receiving 1275 citations.
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Journal ArticleDOI
Antisense and antigene properties of peptide nucleic acids
Jeffery C. Hanvey,Nancy J. Peffer,John E. Bisi,Stephen A. Thomson,Rodolfo Cadilla,John A. Josey,Daniel J. Ricca,C. Fred Hassman,Michele A. Bonham,Karin G. Au,Stephen G. Carter,David A. Bruckenstein,Ann Boyd,Stewart A. Noble,Lee E. Babiss +14 more
TL;DR: Nuclear microinjection of cells constitutively expressing SV40 large T antigen with either a 15-mer or 20-mer PNA targeted to the T Ag messenger RNA suppressed T Ag expression, and Binding of PNAs to RNA resulted in site-specific termination of both reverse transcription and in vitro translation.
Journal ArticleDOI
Fmoc mediated synthesis of Peptide Nucleic Acids
Stephen A. Thomson,John A. Josey,Rodolfo Cadilla,Micheal D. Gaul,C. Fred Hassman,Michael Joseph Luzzio,Adrian J. Pipe,Kathryn L. Reed,Daniel J. Ricca,Robert W. Wiethe,Stewart A. Noble +10 more
TL;DR: The N-terminal Fmoc group is retained on the final oligomer, following HF cleavage and deprotection, providing a convenient lipophilic handle for HPLC purification.
Journal ArticleDOI
Strand-invasion of duplex DNA by peptide nucleic acid oligomers
TL;DR: Evidence is provided that PNAs can, in fact, bind to their complementary sequence in DNA independent of the DNA-strand polarity--that is, a PNA binds to DNA in both "parallel" and "antiparallel" fashion.
Journal ArticleDOI
An assessment of the antisense properties of RNase H-competent and steric-blocking oligomers.
Michele A. Bonham,Stephen C. Brown,Ann Boyd,Pamela H. Brown,David A. Bruckenstein,Jeffery C. Hanvey,Stephen A. Thomson,Adrian J. Pipe,Fred Hassman,John E. Bisi,Brian C. Froehler,Mark D. Matteucci,Richard W. Wagner,Stewart A. Noble,Lee E. Babiss +14 more
TL;DR: It is concluded that PNAs could be effective antisense agents with additional chemical modification that will lead to more rapid association with their RNA target.
Journal ArticleDOI
Probing structural factors stabilizing antisense oligonucleotide duplexes: NMR studies of a DNA.DNA duplex containing a formacetal linkage.
Xiaolian Gao,Frank K. Brown,Peter W. Jeffs,Norbert Bischofberger,Kuei Ying Lin,Adrian J. Pipe,Stewart A. Noble +6 more
TL;DR: Comparison of parameters such as 2D cross-peak intensities, coupling constants, and spectral patterns indicates that structural perturbations caused by the incorporation of the formacetal linkage are minimal and localized to the central T4.A4 block.