S
Stuart Smith
Researcher at Children's Hospital Oakland Research Institute
Publications - 75
Citations - 6640
Stuart Smith is an academic researcher from Children's Hospital Oakland Research Institute. The author has contributed to research in topics: Fatty acid synthase & Acyl carrier protein. The author has an hindex of 40, co-authored 75 publications receiving 6328 citations. Previous affiliations of Stuart Smith include University of California, San Francisco.
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Journal ArticleDOI
Molecular cloning and sequencing of a cDNA encoding the thioesterase domain of the rat fatty acid synthetase.
TL;DR: The Thioesterase I domain exhibits a low, albeit discernible, homology with the discrete medium-chain S-acyl fatty acid synthetase thioester hydrolases from rat mammary gland and duck uropygial gland, suggesting a distant but common evolutionary ancestry for these proteins.
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Mammalian ACSF3 Protein Is a Malonyl-CoA Synthetase That Supplies the Chain Extender Units for Mitochondrial Fatty Acid Synthesis
TL;DR: The objective of this study was to identify a source of intramitochondrial malonyl-CoA that could be used for de novo fatty acid synthesis in mammalian mitochondria and found the mitochondrial ACSF3 enzyme is capable of filling this role by utilizing free malonic acid as substrate.
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Construction of a cDNA encoding the multifunctional animal fatty acid synthase and expression in Spodoptera frugiperda cells using baculoviral vectors.
A K Joshi,Stuart Smith +1 more
TL;DR: Results indicate that, in the insect cell host, all seven catalytic components of the 2505-residue recombinant fatty acid synthase fold correctly, the acyl-carrier-protein domain is appropriately phosphopantetheinylated post-translationally, and the multifunctional polypeptide forms catalytically competent dimers.
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Compromised mitochondrial fatty acid synthesis in transgenic mice results in defective protein lipoylation and energy disequilibrium.
Stuart Smith,Andrzej Witkowski,Ayesha Moghul,Yuko Yoshinaga,Michael Nefedov,Pieter J. de Jong,Dejiang Feng,Loren G. Fong,Yiping Tu,Yan Hu,Stephen G. Young,Thomas Pham,Carling Cheung,Shana M. Katzman,Martin D. Brand,Casey L. Quinlan,Marcel Fens,Frans A. Kuypers,Stephanie Misquitta,Stephen M Griffey,Son Hai Tran,Afshin Gharib,Afshin Gharib,Jens Knudsen,Hans Kristian Hannibal-Bach,Grace Y. Wang,Sandra Larkin,Jennifer L. Thweatt,Saloni Pasta +28 more
TL;DR: De novo synthesis of precursors for the protein lipoylation pathway plays a vital role in maintenance of mitochondrial function and overall vigor in mice with compromised mitochondrial fatty acid synthesis.
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Head-to-Head Coiled Arrangement of the Subunits of the Animal Fatty Acid Synthase
Andrzej Witkowski,Alokesh Ghosal,Anil K. Joshi,H. Ewa Witkowska,Francisco J. Asturias,Stuart Smith +5 more
TL;DR: The proximity of the N-terminal beta-ketoacyl synthase domains and their essential role in dimerization is consistent with a revised model for the FAS in which a head-to-head arrangement of two coiled subunits facilitates functional interactions between the dimeric beta- ketoacyL synthase and the acyl carrier protein domains of either subunit.