Product enhancement of triterpenoid saponins in cell suspension cultures of Leucas aspera Spreng

Anticancer properties of medicinal plants and their bioactive compounds against breast cancer: a review on recent investigations

Enhanced gymnemic acids production in cell suspension cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm. through elicitation

Secondary metabolites are incontestably key specialized molecules with proven health-promoting effects on human beings. Naturally synthesized secondary metabolites are considered an important source of pharmaceuticals, food additives, cosmetics, flavors, etc., Therefore, enhancing the biosynthesis of these relevant metabolites by maintaining natural authenticity is getting more attention. The application of exogenous jasmonates (JAs) is well recognized for its ability to trigger plant growth and development. JAs have a large spectrum of action that covers seed germination, hypocotyl growth regulation, root elongation, petal expansion, and apical hook growth. This hormone is considered as one of the key regulators of the plant’s growth and development when the plant is under biotic or abiotic stress. The JAs regulate signal transduction through cross-talking with other genes in plants and thereby deploy an appropriate metabolism in the normal or stressed conditions. It has also been found to be an effective chemical elicitor for the synthesis of naturally occurring secondary metabolites. This review discusses the significance of JAs in the growth and development of plants and the successful outcomes of jasmonate-driven elicitation of secondary metabolites including flavonoids, anthraquinones, anthocyanin, xanthonoid, and more from various plant species. However, as the enhancement of these metabolites is essentially measured via in vitro cell culture or foliar spray, the large-scale production is significantly limited. Recent advancements in the plant cell culture technology lay the possibilities for the large-scale manufacturing of plant-derived secondary metabolites. With the insights about the genetic background of the metabolite biosynthetic pathway, synthetic biology also appears to be a potential avenue for accelerating their production. This review, therefore, also discussed the potential manoeuvres that can be deployed to synthesis plant secondary metabolites at the large-scale using plant cell, tissue, and organ cultures.

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Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

Green synthesis, characterization, photoluminescence and biological studies of silver nanoparticles from the leaf extract of Muntingia calabura.

Citrullus colocynthis (L.) Schard (Cucurbitaceae) is an endangered medicinal herb. It contains glycosides, cucurbitacins (colocynthin and colocynthetin), cucurbitacins A, B, C, D, E, I, J, K and L which are having medicinal values in various parts of the plant. Cucurbitacin E, a highly oxidated steroid consisting of a tetracyclic triterpene which is more potential triterpenoid for anti-cancer. In this study, we made an attempt to investigate the effect of elicitors with methyl jasmonate (MeJA), salicylic acid (SA), precursors like squalene and mevalonic acid as abiotic elicitors. Precursors were used for enhancement production of cucurbitacin E through cell suspension culture and in vitro plant cultures of Citrullus colocynthis. Based on HPLC results, MeJA is better elicitor than SA and in precursors squalene is better than mevalonic acid for cucurbitacin E production from Citrullus colocynthis cell suspension and in vitro plant cultures. Among all the concentration of elicitors MeJA, SA and precursors squalene, mevalonic acid, 75 µM concentrations have shown best results and MeJA showed highest peak area of 17.97 with 8.63 fold in cell suspension culture. Whereas, the effect of elicitor and precursor feeding studies in in vitro plant culture have produced high amount of cucurbitacin E at 100 µM concentration. Hence, the protocol established in the present study provides an opportunity for enhancement of biological active (anticancer) compound cucurbitacin E.

Enhancement of production of pharmaceutically important anti-cancerous compound; cucurbitacin E via elicitation and precursor feeding of in vitro culture of Citrullus colocynthis (L.) Schard

In Vitro Anti-Inflammatory Activity of Green Synthesized Silver Nanoparticles and Leaf Methanolic Extract of Solanum khasianum Clarke

Abstract Background Solanum khasianum is an important medicinal herb of the Solanaceae family. The present study was focused to determine the bioactive compounds in S. khasianum leaf and root extract by GC–MS analysis and their antibacterial activity by agar well diffusion method. Results Sixteen bioactive compounds were detected in leaf extract and thirty-two compounds in root methanolic extract by GC–MS. The major potent compounds identified in leaf and root extracts were heptadecane 9-hexyl (43.65%) and stigmasterol (23.18%). The root extract showed increased antibacterial activity than leaf extract. Conclusion These extracts possessed significant antibacterial activity against the tested bacterial isolates in dose-dependent manner. This study provides the phytoconstituents, antibacterial property and scientific evidence for the traditional claim and use of S. khasianum. 

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GC–MS profiling and antibacterial activity of Solanum khasianum leaf and root extracts

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Sunitha Dharavath

Papers

Enhancement of production of pharmaceutically important anti-cancerous compound; cucurbitacin E via elicitation and precursor feeding of in vitro culture of Citrullus colocynthis (L.) Schard

Green synthesis, characterization, photoluminescence and biological studies of silver nanoparticles from the leaf extract of Muntingia calabura.

In Vitro Anti-Inflammatory Activity of Green Synthesized Silver Nanoparticles and Leaf Methanolic Extract of Solanum khasianum Clarke

GC–MS profiling and antibacterial activity of Solanum khasianum leaf and root extracts

GC–MS profiling and antibacterial activity of Solanum khasianum leaf and root extracts