Product enhancement of triterpenoid saponins in cell suspension cultures of Leucas aspera Spreng

Anticancer properties of medicinal plants and their bioactive compounds against breast cancer: a review on recent investigations

Enhanced gymnemic acids production in cell suspension cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm. through elicitation

Secondary metabolites are incontestably key specialized molecules with proven health-promoting effects on human beings. Naturally synthesized secondary metabolites are considered an important source of pharmaceuticals, food additives, cosmetics, flavors, etc., Therefore, enhancing the biosynthesis of these relevant metabolites by maintaining natural authenticity is getting more attention. The application of exogenous jasmonates (JAs) is well recognized for its ability to trigger plant growth and development. JAs have a large spectrum of action that covers seed germination, hypocotyl growth regulation, root elongation, petal expansion, and apical hook growth. This hormone is considered as one of the key regulators of the plant’s growth and development when the plant is under biotic or abiotic stress. The JAs regulate signal transduction through cross-talking with other genes in plants and thereby deploy an appropriate metabolism in the normal or stressed conditions. It has also been found to be an effective chemical elicitor for the synthesis of naturally occurring secondary metabolites. This review discusses the significance of JAs in the growth and development of plants and the successful outcomes of jasmonate-driven elicitation of secondary metabolites including flavonoids, anthraquinones, anthocyanin, xanthonoid, and more from various plant species. However, as the enhancement of these metabolites is essentially measured via in vitro cell culture or foliar spray, the large-scale production is significantly limited. Recent advancements in the plant cell culture technology lay the possibilities for the large-scale manufacturing of plant-derived secondary metabolites. With the insights about the genetic background of the metabolite biosynthetic pathway, synthetic biology also appears to be a potential avenue for accelerating their production. This review, therefore, also discussed the potential manoeuvres that can be deployed to synthesis plant secondary metabolites at the large-scale using plant cell, tissue, and organ cultures.

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Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

Green synthesis, characterization, photoluminescence and biological studies of silver nanoparticles from the leaf extract of Muntingia calabura.

Citrullus colocynthis (L.) Schard (Cucurbitaceae) is an endangered medicinal herb. It contains glycosides, cucurbitacins (colocynthin and colocynthetin), cucurbitacins A, B, C, D, E, I, J, K and L which are having medicinal values in various parts of the plant. Cucurbitacin E, a highly oxidated steroid consisting of a tetracyclic triterpene which is more potential triterpenoid for anti-cancer. In this study, we made an attempt to investigate the effect of elicitors with methyl jasmonate (MeJA), salicylic acid (SA), precursors like squalene and mevalonic acid as abiotic elicitors. Precursors were used for enhancement production of cucurbitacin E through cell suspension culture and in vitro plant cultures of Citrullus colocynthis. Based on HPLC results, MeJA is better elicitor than SA and in precursors squalene is better than mevalonic acid for cucurbitacin E production from Citrullus colocynthis cell suspension and in vitro plant cultures. Among all the concentration of elicitors MeJA, SA and precursors squalene, mevalonic acid, 75 µM concentrations have shown best results and MeJA showed highest peak area of 17.97 with 8.63 fold in cell suspension culture. Whereas, the effect of elicitor and precursor feeding studies in in vitro plant culture have produced high amount of cucurbitacin E at 100 µM concentration. Hence, the protocol established in the present study provides an opportunity for enhancement of biological active (anticancer) compound cucurbitacin E.

Enhancement of production of pharmaceutically important anti-cancerous compound; cucurbitacin E via elicitation and precursor feeding of in vitro culture of Citrullus colocynthis (L.) Schard

In Vitro Anti-Inflammatory Activity of Green Synthesized Silver Nanoparticles and Leaf Methanolic Extract of Solanum khasianum Clarke

Plants are excellent sources of secondary metabolites that have been used for the treatment of human diseases. The plant Momordica cymbalaria contains different types of phytochemicals like phenols, steroids, flavonoids, alkaloids and tannins. The present investigation was carried out to determine the bioactive compounds present in the different plant parts of Momordica cymbalaria by Gas chromatography- Mass spectroscopy (GC-MS) technique. Chromatograms and bioactive compounds of in vivo leaf, root and in vitro leaf callus methanolic extracts of Momordica cymbalaria are derived by GC-MS technique. 37 compounds were found in leaf methanolic extracts, 20 compounds in vitro leaf callus methanolic extracts and 18 compounds in root methanolic extracts. These are important bioactive compounds n- Hexadecanoic acid, 9-octadecenoic acid methyl ester, Cholesterol, Cholestanol, 5β-cholestane-3α,7α,12α,24α,25-pentol TMS, 1-Heptatriacotanol, Vitamin E, Ethyl iso-allocholate, Stigmasterol, Β-sitosterol, Lupeol, Diethyl Phthalate, Propanoic acid,2-(3-acetoxy-4,4,14-trimethyl and rost-8-en-17-yl), 1H-pyrrole-2,5,dihydro-1-nitroso, d-Mannose, Spirost-8-en-11-one,3-hydroxy, (3β,5α,14 β,20β,22 β,25R) these are determined from in vivo leaf, root and in vitro callus explants. These identified compounds possess biological activities which can act against the incurable human diseases.

https://www.plantsjournal.com/archives/2021/vol9issue3/Partc/9-3-9-141.pdf

GC-MS analysis of bioactive compounds in the plant parts of methanolic extracts of Momordica cymbalaria Fenzl

The study aimed to identify bioactive compounds in Muntingia calabura leaf and root methanolic extracts. The Gas Chromatography and Mass Spectroscopy (GC-MS) technique were used to identify bioactive compounds. GC-MS analysis revealed 38 compounds in the leaf and 15 compounds in the root methanolic extracts of M. calabura. The prime potent compound found in leaf extract is 2-{3-[(E)-2-(1H-indol-3-yl)ethenyl]-1,2,4-oxadiazol-5-yl}phenol with 5.78% peak area and cholest-4-en-6-on-3-ol is found in root extracts, has the highest 63.7% peak area and another potent compound Lupeol has 7.3% peak area. The bioactive compounds identified in M. calabura have antibacterial activity against various bacterial strains such as gram-positive and gram-negative bacteria, which showed the efficacy of in vivo plant extracts. These findings validate the therapeutic potentiality of M. calabura leaf and root samples. Furthermore, these screened potential bioactive compounds can be used effectively for biomedical and therapeutic applications.

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Gas Chromatographic analysis of potentially bioactive compounds in leaf and root extracts of Muntingia calabura and their expected antibacterial activities

Suvarchala Vankudoth

Papers

Enhancement of production of pharmaceutically important anti-cancerous compound; cucurbitacin E via elicitation and precursor feeding of in vitro culture of Citrullus colocynthis (L.) Schard

Green synthesis, characterization, photoluminescence and biological studies of silver nanoparticles from the leaf extract of Muntingia calabura.

In Vitro Anti-Inflammatory Activity of Green Synthesized Silver Nanoparticles and Leaf Methanolic Extract of Solanum khasianum Clarke

GC-MS analysis of bioactive compounds in the plant parts of methanolic extracts of Momordica cymbalaria Fenzl

Gas Chromatographic analysis of potentially bioactive compounds in leaf and root extracts of Muntingia calabura and their expected antibacterial activities