Susan Isaac

TL;DR: The method described represents a highly efficient and reliable technique for protoplast production from Aspergillus nidulans and corresponded to both the point of maximum autolysis and maximum lytic enzyme activity.

TL;DR: The anatomy of diseased stems from brooms induced on cocoa by the fungus Crinipellis perniciosa was examined using the scanning electron microscope, and the amounts of selected cytokinins were measured by immunoassay.

TL;DR: The method developed was specific to chitin, and significantly more sensitive than previously described methods, and sufficiently sensitive to measure a minimum fungal biomass in cocoa of 40 ng mg −1 D.W. of plant tissue.

TL;DR: Although there was little relationship between glasshouse and field-trial results, under field conditions one of the somaclones had a mean incidence of disease, as estimated by transformed data, of 1·9 compared with 37·9 for the most susceptible somaclone and 3·5 for Bolero, the most resistant commercial cultivar.

TL;DR: The symptoms of witches' broom disease in cocoa, caused by the Basidiomycete fungus Crinipellis perniciosa, are pronounced swelling of the terminal and axillary buds followed in the long term by necrosis of this tissue.