Showing papers by "Syafiqah Saidin published in 2020"

Size Preferences Uptake of Glycosilica Nanoparticles to MDA-MB-231 Cell.

Abstract: Recently, studies on the development and investigation of carbohydrate-functionalized silica nanoparticles (NPs) and their biomedicine applications such as cell-specific targeting and bioimaging has been carried out extensively. Since the number of breast cancer patients has been growing in recent years, potential NPs were being studied in this project for targeting breast cancer cells. Mannose receptors can be found on the surface of MDA-MB-231, which is a kind of human breast cancer cell line. Therefore, we decorated a cyanine 3 fluorescent dye (Cy3) and mannosides on the surface of silica NPs for the purpose of imaging and targeting. Galactoside was also introduced onto the surface of silica NPs acting as a control sample. Various sizes of silica NPs were synthesized by using different amounts of ammonium to investigate the effect of the size of NPs on the cellular uptake rate. The physical properties of these NPs were characterized by scanning electron microscope, dynamic light scattering, and their zeta potential. Cellular experiments demonstrated that mannoside-modified NPs can be uptaken by MDA-MB-231. From the experiment, we found out that the best cellular uptake rate of nanoparticle size is about 250 nm. The MTT assay showed that Man@Cy3SiO2NPs are not cytotoxic, indicating they may have the potential for biomedical applications.

Properties of Tilapia Collagen as a Biomaterial for Tissue Engineering: A Review

Abstract: Collagen is one of the common biopolymers used as a biomaterial in tissue engineering applications due to its biocompatibility and biodegradability properties. Collagen derived from various sources and mostly found in porcine and bovine skin. However, due to religious concerns and the dangers of animal-borne diseases possesses by these mammalian derived collagens, marine collagens are extensively investigated as an alternative to substitute mammalian collagens in tissue engineering applications. Among those marine collagens, tilapia (Oreochromis niloticus) collagens are known to have a great potential to be used as biomaterials for tissue engineering application due to its higher thermal stability compared to other marine sources. Therefore, this study aims to review chemical and biological properties of tilapia collagen as a biomaterial for tissue engineering applications. The contents are mainly focused on the extraction yield, amino acid composition, thermal stability, cross-linking, biocompatibility, biodegradability, immunogenicity and hemostasis of tilapia collagen.

Effects of different polyaniline emeraldine compositions in electrodepositing ginsenoside encapsulated poly(lactic-co-glycolic acid) microcapsules coating: Physicochemical characterization and in vitro evaluation.

Abstract: Even though drug-eluting stent (DES) has prominently reduced restenosis, however, its complication of delayed endothelialization has caused chronic side effect. A coating of ginseng-based biodegradable polymer could address this issue due to its specific therapeutic values. However, deposition of this type of stable coating on metallic implant often scarce. Therefore, in this study, different polyaniline (PANI) emeraldine compositions were adopted to electrodeposit ginsenoside encapsulated poly(lactic-co-glycolic acid) microcapsules coating. The coating surfaces were analyzed using attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, contact angle, and atomic force microscopy instruments. A month coating stability was then investigated with an evaluation of in vitro human umbilical vein endothelial cell analyses consisted of cytotoxicity and cells attachment assessments. The 1.5 mg PANI emeraldine has assisted the formation of stable, uniform, and rounded microcapsules coating with appropriate wettability and roughness. Less than 1.5 mg PANI emeraldine was not enough to drive the formation of microcapsules coating while greater than 1.5 mg caused the deposition of melted microcapsules. The similar coating also has promoted greater cells proliferation and attachment compared to other coating variation. Therefore, the utilization of electrodeposition to deposit a drug-based polymer coating could be implemented to develop DES, in accordance to stent implantation which ultimately aims for enrich endothelialization.

Design and Characterization of Impedance based E. Coli Sensor

Abstract: Foodborne illness has become a concern due to high disease rate and huge losses caused. It is critical to detect and identify the pathogen in food by using sensor. However the conventional methods for pathogen detection are quite time-consuming, costly and label-dependent. This paper presents the design, fabrication and characterization of the impedance based biosensor for the detection of E. coli in water with features of low cost, rapid detection, easy to use and label-free. The interdigital electrodes and microfluidic system based devices were tested in different concentrations of E. coli samples with different structure electrode parameters for sensor characterization. Impedance analyzer was used for monitoring the impedance change to determine the operating frequency. The fabricated interdigital electrodes are also able to discriminate between dry and wet conditions by presenting different impedance outcomes at low frequency. The results depicted in this paper provide a guideline for detection of E. coli contamination level at different concentration with corresponding impedance range.