https://www.cell.com/cell/pdf/0092-8674(92)90610-O.pdf

Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA

Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold

https://www.cell.com/cell/pdf/0092-8674(80)90409-2.pdf

The nuclear envelope lamina is reversibly depolymerized during mitosis

Human endothelial cells, obtained by collagenase treatment of term umbilical cord veins, were cultured using Medium 199 supplemented with 20% fetal calf serum. Small clusters of cells initially spread on plastic or glass, coalesced and grew to form confluent monolayers of polygonal cells by 7 days. Cells in primary and subcultures were identified as endothelium by the presence of Weibel-Palade bodies by electron microscopy. A morphologically distinct subpopulation of cells contaminating some primary endothelial cultures was selectively subcultured, and identified by ultrastructural criteria as vascular smooth muscle. Autoradiography of endothelial cells after exposure to [3H]thymidine showed progressive increases in labeling in growing cultures beginning at 24 h. In recently confluent cultures, labeling indices were 2.4% in central closely packed regions, and 53.2% in peripheral growing regions. 3 days after confluence, labeling was uniform, being 3.5 and 3.9% in central and peripheral areas, respectively. When small areas of confluent cultures were experimentally "denuded," there were localized increases in [3H]thymidine labeling and eventual reconstitution of the monolayer. Liquid scintillation measurements of [3H]thymidine incorporation in primary and secondary endothelial cultures in microwell trays showed a similar correlation of DNA synthesis with cell density. These data indicate that endothelial cell cultures may provide a useful in vitro model for studying pathophysiologic factors in endothelial regeneration.

/pdf/human-vascular-endothelial-cells-in-culture-growth-and-dna-547gh0526i.pdf

HUMAN VASCULAR ENDOTHELIAL CELLS IN CULTURE Growth and DNA Synthesis

Sera from patients with scleroderma contained several autoantibodies to nuclear antigens which were distinguished by different patterns of nuclear immunofluorescence staining. One of these autoantibodies reacted with centromeric regions of chromosomes. In chromosome spreads, the staining appeared as two small spheres at the centromere, resembling kinetochores. The antigenic determinant appeared to be a protein or polypeptide tightly bound to DNA. The autoantibody was reactive with centromeres of cells derived from humans, mice, and Chinese hamsters. The autoantibody was present in high frequency in the calcinosis/Raynaud's phenomenon/esophageal dysmotility/sclerodactyly/telangiectasia variant (CREST) of scleroderma.

https://www.pnas.org/content/pnas/77/3/1627.full.pdf

T.A. Okada

Papers

The mechanism of C- and G-banding of chromosomes

Nuclear proteins. III. The fibrillar nature of the nuclear matrix.

Association of chromatin fibers with the annuli of the nuclear membrane.

Fine structure of kinetochore in Indian muntjac.

Electron microscopy of human fibroblasts in tissue culture during logarithmic and confluent stages of growth.