Showing papers in "Experimental Cell Research in 1976"

TL;DR: A clonal cell line exhibiting many of the properties of skeletal muscle has been derived from embryonic BDIX rat heart tissue and electron microscopic analysis reveals that the myotubes contain elaborately branched tubular systems and numerous bundles of thick filaments with distinct M-bands.

TL;DR: A hypothesis of contact guidance which states that the shape of the substratum imposes mechanical restrictions on the formation of certain linear bundles of microfilaments which are involved in cell locomotion was used to predict fibroblast behaviour on prism-shaped substrata.

TL;DR: The results support earlier reports that the expression of human ribosomal RNA (rRNA) genes is suppressed in mouse-human hybrid cells and suggest that silver staining by the Ag-AS method reflects activity of rRNA genes rather than just the presence of these genes.

TL;DR: It was calculated that a significant amount of the cathepsin D activity in the liver is present in the Kupffer cells, and nearly equal specific activities for the lysosomal enzyme acid phosphatase were shown in purified K upffer cells.

TL;DR: It is concluded that these cell lines proliferate as myoblasts and develop into cells which phenotypically resemble smooth muscle.

TL;DR: Serum prepared from cell-free plasma had a considerably lower activity than serum prepared from whole blood, but the major part of the growth-promoting activity of serum could be ascribed to a platelet factor released during the coagulation process.

TL;DR: It is suggested that cells possess a membrane reserve in their microvilli and other protuberances which can be utilized for spreading and initiating cell locomotion.

TL;DR: In this paper, differential staining techniques based on the incorporation of bromodeoxyuridine (BUdR) into DNA permits the identification of metaphase cells which have replicated once, twice, and three or more times.

TL;DR: Evidence is presented which suggests that the particles are an in vivo structural repeating unit of deoxyribonucleoprotein, and not a preparation-induced artefact.

TL;DR: The CB-induced rearrangement of microfilament bundles occurs only in vivo and not in glycerinated cell contractility models.

TL;DR: The matrix may play a role in the arrangement of chromatin into the chromomeres of meiotic and mitotic chromosomes and several observations suggest DNA is attached to the matrix at many sites throughout the nucleus.

TL;DR: It is demonstrated, using ultrathin sections of Epon embedded cells, that after 2 or 5 min of labeling the radioactivity is predominantly associated with perichromatin fibrils localized frequently in proximity to condensed chromatin regions.

TL;DR: The observed relationship between occurrence of well developed ruffling membranes, macro-pinocytosis and cell multiplication indicates that one of the functions of growth-promoting factors may be stimulation of plasma membrane turnover.

TL;DR: In this paper, the enzymatic activity of SOD-1 in erythrocytes has been studied in several cases of partial monosomies and full and partial trisomies 21.

TL;DR: The system described herein offers a favourable preparation for revealing the ultrastructural organization of microtubules and there are reasons to believe that Zn 2+ interferes with the closing process of micro Tubulin so that more protofilaments are added in the assembly process than during normal conditions.

TL;DR: Ammonia (10 mM NH4Cl) partially inhibited protein degradation in isolated rat hepatocytes, but an electron microscopic, morphometric analysis revealed that the inhibition was accompanied by lysosomal vacuolization, i.e. a large increase in the volume fraction of more and less electronlucent vacuoles containing remnants of digested material.

TL;DR: It was found that the stainability of cells after drug exposure depended on the cell line used and the particular drug used, and cell survival as measured by cloning efficiency did not correlate with survival measured by any of the other methods.

TL;DR: Time-lapse cinematographic analysis of clones of human diploid fibroblasts indicate heterogeneity in clonal division behaviour, and a consistent division pattern at all population doubling levels is one of low average inter division time for early and late generations of a clone and high average interdivision time for the middle range of generations ofA clone.

TL;DR: Results show that cytoplasm of the posterior region of the Drosophila egg contains germ-cell determinants which function autonomously after transplantation to a somatic, i.e. mid-ventrals of the egg, and that pole cells either formed in or transferred to this ectopic region can still reach the gonads and contribute to the germ line.

TL;DR: The mouse spermatid nucleus becomes resistant to sonication at step 12, resistant to digestion by trypsin-DNase at step 15 and resistant to lysis by SDS between the testis and the caput epididymis, correlated with changes in the basic nuclear proteins.

TL;DR: Although the initial rate of BHK cell attachment is faster in serum-free medium than serum-containing medium, no cell spreading occurs in serum -free medium; however, only ConA results in normal morphology of cell spreading.

TL;DR: There is a marked shift in the nuclear size distribution of the slow or non-replicating cell population to larger sizes with increasing in vitro passage.

TL;DR: A factor required for spreading of substratum-attached baby hamster kidney cells, Chinese hamster ovary cells, HeLa cells, and L cells has been isolated and purified from fetal calf serum and antibody against the fetal calf spreading factor induced cell spreading.

TL;DR: It is concluded that human umbilical cord vein endothelial cells exhibit a stringent pattern of growth control in vitro since confluent cultures do not respond to serum growth factors and may be necessary for the maintenance and function of blood vessels in vivo.

TL;DR: Axenic late log phase cultures of Tetrahymena pyriformis DN-B3 are deciliated by treatment with dibucaine, suggesting that underlying gradients, perhaps related to Ca2+, are significant in the deciliation process.

TL;DR: It is proposed that thrombin may play a role in wound healing by stimulating proliferation of not only fibroblasts but also B lymphocytes, a process of possible physiological significance in defending the host against pathogens and during inflammations.

TL;DR: Isolated hepatocytes prepared under hypoxic conditions are functional as demonstrated by their ability to carry out gluconeogenesis under normal and stimulated conditions, by the selectivity of the cell membrane towards various substrates and by the preservation of insulin receptors on the cell membranes.

TL;DR: A normal serum protein, purified by gel filtration and ion exchange chromatography, enhanced the chemotactic response of mouse peritoneal macrophages to EAMS (endotoxin-activated mouse serum) and increased the number of migrated macrophage in the absence of EAMS.

TL;DR: The eggs of Xenopus laevis are capable of initiating spindle formation and cleavage in response to microinjection of partially purified components of sea urchin sperm, suggesting the centriole and basal plate as the active materials.

TL;DR: The radial orientation of axons on unoriented substrata was modified by a tendency for the axons to follow the aligned collagen fibrils during extension by contact guidance, and the results are suggested to be significant for extending axons and migrating cells on extracellular substrata in vivo.