Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes.

Publisher Summary This chapter describes the malondialdehyde (MDA) as index of lipid peroxidation. The determination of malondialdehyde (MDA) has attracted widespread interest, because it appears to offer a facile means of assessing lipid peroxidation in biological materials. Malondialdehyde occurs in biological materials in free state and in various covalently bound forms. Urine also contains small amounts of MDA adducts with guanine, the phospholipid bases serine and ethanolamine, and other unidentified reactants. Free MDA is a minor and variable excretory product. It is apparent from the occurrence of these derivatives in urine that MDA forms adducts with proteins, nucleic acids, and other substances in vivo, and this compromises the assessment of lipid peroxidation in the tissues based on the determination of free MDA. The pH required for maximum yield of MDA varies among biological materials depending on the nature of the derivatives present. MDA may be generated during hydrolysis by the oxidation of polyunsaturated fatty acids (PUFA) in the sample and by the degradation of preexisting oxidation products. Pigments present in the sample, or generated during hydrolysis, also can interfere in the colorimetric assessment of MDA. These problems, and possibilities for their resolution, are discussed in the chapter.

Malondialdehyde determination as index of lipid peroxidation

Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury.

Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems.

Quantification of urban organic aerosols at a molecular level: Identification, abundance and seasonal variation

The 3-Methyl-2-benzothiazolone Hydrazone Test. Sensitive New Methods for the Detection, Rapid Estimation, and Determination of Aliphatic Aldehydes

Application of Thin Layer Chromatography to the Analysis of Atmospheric Pollutants and Determination of Benzo(a)pyrene.

Comparison of fifty-two spectrophotometric methods for the determination of nitrite

Comparison of Spectrophotometric and Spectrophotofluorometric Methods for the Determination of Malonaldehyde.

Mass Spectra of Aliphatic Thiols and Sulfides

T. W. Stanley

Papers

The 3-Methyl-2-benzothiazolone Hydrazone Test. Sensitive New Methods for the Detection, Rapid Estimation, and Determination of Aliphatic Aldehydes

Application of Thin Layer Chromatography to the Analysis of Atmospheric Pollutants and Determination of Benzo(a)pyrene.

Comparison of fifty-two spectrophotometric methods for the determination of nitrite

Comparison of Spectrophotometric and Spectrophotofluorometric Methods for the Determination of Malonaldehyde.

Mass Spectra of Aliphatic Thiols and Sulfides