T
Takanori Kigawa
Researcher at RIKEN Quantitative Biology Center
Publications - 243
Citations - 9181
Takanori Kigawa is an academic researcher from RIKEN Quantitative Biology Center. The author has contributed to research in topics: Domain (software engineering) & Protein structure. The author has an hindex of 49, co-authored 238 publications receiving 8476 citations. Previous affiliations of Takanori Kigawa include Tokyo Institute of Technology & University of Tokyo.
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Journal ArticleDOI
Cell-free production and stable-isotope labeling of milligram quantities of proteins
Takanori Kigawa,Takashi Yabuki,Yasuhiko Yoshida,Michio Tsutsui,Yutaka Ito,Takehiko Shibata,Shigeyuki Yokoyama +6 more
TL;DR: Creatine phosphate and creatine kinase were used as the energy source regeneration system, and the other components of the reaction mixture were optimized to improve the productivity of an Escherichia coli cell‐free protein synthesis system.
Journal ArticleDOI
Role of the ENTH Domain in Phosphatidylinositol-4,5-Bisphosphate Binding and Endocytosis
Toshiki Itoh,Seizo Koshiba,Takanori Kigawa,Akira Kikuchi,Shigeyuki Yokoyama,Tadaomi Takenawa +5 more
TL;DR: A strong affinity of the ENTH domain for phosphatidylinositol-4,5-bisphosphate [PtdIns( 4,5)P2] is demonstrated, which is essential for endocytosis mediated by clathrin-coated pits.
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Preparation of Escherichia coli cell extract for highly productive cell-free protein expression.
Takanori Kigawa,Takashi Yabuki,Natsuko Matsuda,Takayoshi Matsuda,Rie Nakajima,Akiko Tanaka,Shigeyuki Yokoyama +6 more
TL;DR: A high-throughput pipeline for protein sample preparation for structural genomics and proteomics by using cell-free protein synthesis using PCR-amplified templates and large-scale protein production for structure determinations is established.
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An unnatural base pair for incorporating amino acid analogs into proteins
Ichiro Hirao,Takashi Ohtsuki,Tsuyoshi Fujiwara,Tsuneo Mitsui,Tomoko Yokogawa,Taeko Okuni,Hiroshi Nakayama,Koji Takio,Takashi Yabuki,Takanori Kigawa,Koichiro Kodama,Takashi Yokogawa,Kazuya Nishikawa,Shigeyuki Yokoyama +13 more
TL;DR: This coupled transcription–translation system will permit the efficient synthesis of proteins with a tyrosine analog at the desired position in an Escherichia coli cell-free system.
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Structural genomics projects in Japan.
Shigeyuki Yokoyama,Hiroshi Hirota,Takanori Kigawa,Takashi Yabuki,Mikako Shirouzu,Takaho Terada,Yutaka Ito,Yo Matsuo,Yutaka Kuroda,Yoshifumi Nishimura,Yoshimasa Kyogoku,Kunio Miki,Ryoji Masui,Seiki Kuramitsu +13 more
TL;DR: Two major structural genomics projects exist in Japan and one focuses on human membrane proteins and the other focuses on bacterial, mammalian, and plant protein structures by X-ray crystallography and NMR spectroscopy.