Tammo Diercks

TL;DR: The physical characterization of detergent-solubilized protein indicates that phosphate-containing detergents are more efficient in extracting, solubilizing and maintaining Cai in a monomeric state, yet their capacity to ensure protein activity, reconstitution, helix packing, and high-quality NMR spectra was inferior to that of milder deterGents.

TL;DR: In this article , the binding of intrinsically disordered proteins to globular ones can require the folding of motifs into α-helices by concatenating glutamine side chain to main chain hydrogen bonds recently discovered in polyglutamine helices.

TL;DR: NMR assignment and backbone dynamics of the C-terminal domain of colicin A in solution identify ten α-helices that match those observed in the crystal structure, while the 15N{1H} NOEs show differential fast mobility for some of the inter-helical loops and the chain ends.

TL;DR: A minimal fluorination scheme is presented, and the concept on di-(4-fluoro)-heptanoylphosphatidylcholine micelles and solubilised seven-helix transmembrane pSRII protein is illustrated.