Chemotherapy is one of the major treatment methods for cancer However, failure in chemotherapy is not uncommon, mainly due to dose-limiting toxicity associated with drug resistance Management of drug resistance is important towards successful chemotherapy There are many reports in the Chinese literature that natural products can overcome cancer cell drug resistance, which deserve sharing with scientific and industrial communities We summarized the reports into four categories: (1) in vitro studies using cell line models; (2) serum pharmacology; (3) in vivo studies using animal models; and (4) clinical studies Fourteen single compounds were reported to have antidrug resistance activity for the first time In vitro, compounds were able to overcome drug resistance at nontoxic or subtoxic concentrations, in a dose-dependent manner, by inhibiting drug transporters, cell detoxification capacity, or cell apoptosis sensitivity Studies in vivo showed that single compounds, herbal extract, and formulas had potent antidrug resistance activities Importantly, many single compounds, herbal extracts, and formulas have been used clinically to treat various diseases including cancer The review provides comprehensive data on use of natural compounds to overcome cancer cell drug resistance in China, which may facilitate the therapeutic development of natural products for clinical management of cancer drug resistance

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Overcome Cancer Cell Drug Resistance Using Natural Products

Quality assessment of Fritillariae Thunbergii Bulbus sold in Taiwan markets using a validated HPLC-UV method combined with hierarchical clustering analysis.

Verticine is the major bioactive constituent of Fritillaria as a kind of Traditional Chinese Medicine. Pharmacological researches have reported various benefits of verticine, including anticancer, anti-inflammatory, protecting against acute lung injury, tracheobronchial relaxation, antitussive, expectorant, sedative, and analgesic activities, in addition to inhibiting proliferation of cultured orbital fibroblast, angiotensin converting enzyme (ACE), and acetylcholinesterase (AChE) and inhibiting hERG potassium channels. The underlying mechanisms of verticine are still under investigation. This review will comprehensively summarize the metabolism, biological activities, and possible mechanism of verticine.

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Pharmacological Effects of Verticine: Current Status

Breast cancer (BC) is the second leading cause of death among women, and it has become a global health issue due to the increasing number of cases. Different treatment options, including radiotherapy, surgery, chemotherapy and anti-estrogen therapy, aromatase inhibitors, anti-angiogenesis drugs, and anthracyclines, are available for BC treatment. However, due to its high occurrence and disease progression, effective therapeutic options for metastatic BC are still lacking. Considering this scenario, there is an urgent need for an effective therapeutic strategy to meet the current challenges of BC. Natural products have been screened as anticancer agents as they are cost-effective, possess low toxicity and fewer side effects, and are considered alternative therapeutic options for BC therapy. Natural products showed anticancer activities against BC through the inhibition of angiogenesis, cell migrations, proliferations, and tumor growth; cell cycle arrest by inducing apoptosis and cell death, the downstream regulation of signaling pathways (such as Notch, NF-κB, PI3K/Akt/mTOR, MAPK/ERK, and NFAT-MDM2), and the regulation of EMT processes. Natural products also acted synergistically to overcome the drug resistance issue, thus improving their efficacy as an emerging therapeutic option for BC therapy. This review focused on the emerging roles of novel natural products and derived bioactive compounds as therapeutic agents against BC. The present review also discussed the mechanism of action through signaling pathways and the synergistic approach of natural compounds to improve their efficacy. We discussed the recent in vivo and in vitro studies for exploring the overexpression of oncogenes in the case of BC and the current status of newly discovered natural products in clinical investigations.

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A Review of Twenty Years of Research on the Regulation of Signaling Pathways by Natural Products in Breast Cancer

Objective To explore the effect and mechanism of reversing multi-drug resistance(MDR) in adenocarcinoma line A549/DDP by peimineMethods The A549/DDP cells were divided into two groupsThe cells in control were given different concentration of cisplatin,and the cells in expremental group were given 400 μg/mL peimine on the control group basisMTT assay was used to detect the cell proliferation ratio,and IC50 and reversing multipe were calculatedThe A549/DDP cells were divided into blank control group(nothing),control group(cisplatin) and experimental group(cisplatin+400 μg/mL peimine)After 48 h,apoptosis was anlysed by flow cytometry,expression of resistance associated protein LRP was detected by cell immunofluorescence techniqueResults IC50 of cisplatin in experimental group was 415μg/mL,and 1546 μg/mL in control group,the reversing multipe was 373After 48 hours,cell apoptosis rate among blank contronl group,contronl group and experimental group was respectively 256±044,135±142,3816±225,and the expression of LRP of each group was(98±192)/HP,(92±19)/HP,(58±13)/HPThere were much more cell apoptosis and less expression were in the experiment group than in the control group(P005)Conclusions Peimine could reverse MDR of A549/DDP cell lineThe mechanism may be reletived with inducing apoptosis and down-regulating LRP expression

Tang Ying-xue

Papers

Efficiency and mechanisms of peimine reversing multi-drug resistance of A549/DDP cell line