Teresa Muiño-Blanco

TL;DR: Ejaculated ram spermatozoa, freed from seminal plasma by a dextran/swim-up procedure and exposed to cold shock, were incubated with ram seminal plasma proteins and analyzed by fluorescence markers and scanning electron microscopy, showing that the dramatic structural damage induced by cooling reverted after incubation with seminal plasma protein.

TL;DR: Impaired function of cold-shocked ram spermatozoa freed from semen plasma could be prevented by addition of semen plasma proteins, resulting in higher maintained viability values, as well as the ability of Vitamin E to improve sperm survival.

TL;DR: The addition of SP proteins to spermatozoa before and/or after cooling is able to minimize cryoinjury effects and the major proteins in ram SP which are able to protect and repair the cold-shock damage to sperm contain fibronectin-II domains.

TL;DR: The addition of SP from good sperm freezers improved the motility and viability of thawed spermatozoa without any influence on MDA production and increased the percentage of penetrated (SP3) and polyspermic oocytes (SP4) with respect to the control.

TL;DR: It is proved that melatonin has a direct effect on ram spermatozoa in the nonreproductive season, which can be explained, at least in part, by the melatonin capacity as a reactive oxygen species scavenger and antioxidant.