Showing papers by "Thomas D. Petes published in 1980"
TL;DR: Recombinant DNA procedures and the yeast transformation technique were used to insert the yeast gene LEU 2 (coding for beta-isopropylmalate dehydrogenase) into the tandem array of ribosomal DNA genes of the yeast Saccharomyces cerevisiae.
316 citations
TL;DR: The present work presents a meta-analysis of yeast chromosomol genes that highlights the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals.
Abstract: PROSPECTIVES AND SUMMARY 845 GENETIC ANALYSIS IN yEAST 846 Tetrad Analysis 846 New Advances in Genetic Analysis 850 Construction of recombinant DNA molecules 850 Identification of cloned genes 850 Yeast transformation 854 ORGANIZATION AND REPLICATION OF THE YEAST CHROMOSOMES 856 Chromosome Structure 858 Chromosome Replication 860 Structure of replicating nuclear DNA molecules 860 Control of DNA synthesis 862 Yeast DNA polymerases .. ... ... ........ ... ..... 864 ANALYSIS OF SPECIFIC CLASSES OF CHROMOSOMAL GENES 864 Structural Analysis of Single-Copy Genes 865 Repeating Chromosomal Genes 866 Yeast ribosomal DNA genes 866 Structure of yeast tRNA genes 867 Other repeating yeast chromosomol genes 868 STRUCTURE AND REPLICATION OF THE 2p.m PLASMIDS 869 CONCLUSIONS AND FUTURE PROSPECTS 871
142 citations
TL;DR: It is concluded that the recombinant plasmid used for transformation carries regulatory information in addition to LEU2 structural information.
Abstract: The repression of beta-isopropylmalate dehydrogenase, the LEU2 gene product, by leucine and leucine plus threonine was unaffected by the transposition of LEU2 from its original locus on chromosome III to a new locus within the ribosomal deoxyribonucleic acid gene cluster on chromosome XII. Since the expression of the LEU2 gene is probably controlled at a pretranslational level, we conclude that the recombinant plasmid used for transformation carries regulatory information in addition to LEU2 structural information.
19 citations