Showing papers by "Thomas V. O'Halloran published in 2008"
TL;DR: It is shown that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan, affording mechanisms for control of adventitious metal redox and substitution chemistry.
Abstract: Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-π interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry.
216 citations
TL;DR: It is shown that apoSOD1 without disulfide is the most facile state for formation of amyloid-like fibrillar aggregates and proposed that facilitation of post-translational modifications is a promising strategy to reduce SOD1 aggregation in the cell.
205 citations
TL;DR: This work has shown that the thioether group of methionine has begun to emerge as an important Cu(I) ligand for trafficking proteins in more oxidizing cellular environments.
Abstract: Over the last decade, cysteine thiolate ligands have been shown to be critical to the Cu(I) (cuprous) binding chemistry of many cytosolic metallochaperone and metalloregulatory proteins involved in copper physiology. More recently, the thioether group of methionine has begun to emerge as an important Cu(I) ligand for trafficking proteins in more oxidizing cellular environments.
202 citations
Patent•
09 Jun 2008TL;DR: In this paper, a pH-responsive delivery mechanism for delivery of nucleic acids, peptides, small molecules, drugs, etc. in vitro and in vivo was proposed, and a stable, polymer-caged liposomes comprising a pH responsive delivery mechanism was provided.
Abstract: The present invention provides liposomal compositions and methods of using such compositions in vitro and in vivo. In particular, the present invention provides stable, polymer-caged liposomes comprising a pH responsive delivery mechanism for delivery of nucleic acids, peptides, small molecules, drugs, etc. in vitro and in vivo.
5 citations