T
Thomas Vogl
Researcher at Weizmann Institute of Science
Publications - 28
Citations - 1646
Thomas Vogl is an academic researcher from Weizmann Institute of Science. The author has contributed to research in topics: Pichia pastoris & Promoter. The author has an hindex of 18, co-authored 28 publications receiving 1224 citations. Previous affiliations of Thomas Vogl include Institute of Molecular Biotechnology & Queensland University of Technology.
Papers
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Journal ArticleDOI
Regulation of Pichia pastoris promoters and its consequences for protein production
Thomas Vogl,Anton Glieder +1 more
TL;DR: The methylotrophic yeast Pichia pastoris provides a strong, methanol inducible promoter of the alcohol oxidase 1 (AOX1) gene, and the regulation of this promoter has been extensively studied in recent years by characterizing cis-acting sequence elements and trans-acting factors, revealing insights into underlying molecular mechanisms.
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Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris.
TL;DR: This optimized CRISPR/Cas9 system allows rapid, marker-less genome engineering in P. pastoris enabling unprecedented strain and metabolic engineering applications and demonstrates targeting efficiencies approaching 100%.
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New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris
TL;DR: The application of Pichia pastoris for biopharmaceutical production is described and the molecular toolbox available for synthetic biology in P. pastoris is discussed.
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A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris.
Thomas Vogl,Lukas Sturmberger,Thomas Kickenweiz,Richard Wasmayer,Christian Schmid,Anna-Maria Hatzl,Michaela Agnes Gerstmann,Julia Pitzer,Marlies Wagner,Gerhard G. Thallinger,Martina Geier,Anton Glieder +11 more
TL;DR: With the synthetic biology toolbox presented here, P. pastoris is now equipped with one of the largest sets of strong and co-regulated promoters of any microbe, moving it from a protein production host to a general industrial biotechnology host.
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Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects.
TL;DR: The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISpr-Cas systems for highly efficient genome editing in other eukaryotic organisms.