Karin Kovar

TL;DR: The new PAOX1 synthetic promoter library constitutes a basic toolbox to fine-tune gene expression in metabolic engineering and sequential induction of protein expression in synthetic biology.

TL;DR: It is confirmed that, although fundamental knowledge is now available, the development of efficient, economically feasible large-scale bioprocesses remains an obstacle to the commercialisation of this promising technology.

TL;DR: This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors.

TL;DR: The use of flow cytometric (FCM) methods for the analysis of microalgae has been explored in this paper, where the authors present the options for implementation of FCM in the development of biotechnological processes detailed in this review are (i) analyzing the chemical composition of biomass, (ii) monitoring cellular enzyme activity and cell viability, and (iii) sorting cells to isolate those overproducing the target compound or for the preparation of axenic cultures.

TL;DR: The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISpr-Cas systems for highly efficient genome editing in other eukaryotic organisms.