Timothy D. Minogue

TL;DR: Ciprofloxacin induces prophage mobilization as well as significant alterations in metabolism, most notably the up-regulation of the tricarboxylic acid cycle and induced mutation facilitate S. aureus persistence and evolution of resistance during antibiotic therapy.

TL;DR: It is shown that the cell density-dependent synthesis of stewartan EPS, governed by the EsaI/EsaR QS system, is required for proper bacterial adhesion and development of spatially defined, 3D biofilms, and epifluorescence microscopic imaging of infected leaf tissue and excised xylem vessels reveals that the bacteria colonize thexylem with unexpected specificity.

TL;DR: In this article, the crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core.

TL;DR: Genetic evidence is presented that EsaR represses the esaR gene under inducer‐limiting conditions, and that addition of inducer promotes rapid, dose‐dependent derepression, and biochemical data establish thatEsaR regulates its own expression by signal‐independent repression and signal‐dependentDerepression.

TL;DR: The focus of this study was to define the mechanism by which EsaR governs the expression of the cps locus, which encodes functions required for stewartan EPS synthesis and membrane translocation, and to describe that RcsA positively controls its own expression.