T
Timothy Ragan
Researcher at Massachusetts Institute of Technology
Publications - 18
Citations - 1174
Timothy Ragan is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Cytometry & Two-photon excitation microscopy. The author has an hindex of 11, co-authored 15 publications receiving 1072 citations. Previous affiliations of Timothy Ragan include University of Illinois at Urbana–Champaign.
Papers
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Journal ArticleDOI
Serial two-photon tomography for automated ex vivo mouse brain imaging
Timothy Ragan,Lolahon R. Kadiri,Kannan Umadevi Venkataraju,Karsten Bahlmann,Jason Sutin,Julian Taranda,Ignacio Arganda-Carreras,Yongsoo Kim,H. Sebastian Seung,Pavel Osten +9 more
TL;DR: An automated method is described that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning, which opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.
Journal ArticleDOI
Multifocal multiphoton microscopy based on multianode photomultiplier tubes.
Ki Hean Kim,Christof Buehler,Karsten Bahlmann,Timothy Ragan,Wei-Chung Allen Lee,Elly Nedivi,Erica L. Heffer,Sergio Fantini,Peter T. C. So +8 more
TL;DR: It is demonstrated that MAPMT-based MMM has imaging depth comparable to SMM with equivalent sensitivity by imaging tissue phantoms, ex vivo human skin specimens based on endogenous fluorophores, and green fluorescent protein (GFP) expressing neurons in mouse brain slices.
Patent
Systems and methods for volumetric tissue scanning microscopy
Peter T. C. So,Bevin P. Engelward,Timothy Ragan,Karsten Bahlmann,Ki Hean Kim,Lily H. Laiho,Hayden Huang +6 more
TL;DR: In this article, the authors present a method for imaging tissue, which includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the regions of interest across a portion of tissue, imaging a plurality of layers of the tissue in a plurality volume of the volume of interest and detecting a fluorescence image of tissue due to said excitation light.
Patent
Multifocal imaging systems and method
TL;DR: In this paper, a multianode photomultiplier tube (MAPMT) is used for high-speed tissue imaging, where the emission photons from the array of excitation foci are collected simultaneously by a MAPMT and the signals from each anode are detected using high sensitivity, low noise single photon counting circuits.
Journal ArticleDOI
High-resolution whole organ imaging using two-photon tissue cytometry
Timothy Ragan,Jeremy D. Sylvan,Ki Hean Kim,Hayden Huang,Karsten Bahlmann,Richard T. Lee,Peter T. C. So +6 more
TL;DR: Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution.