Timothy Ragan

TL;DR: An automated method is described that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning, which opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

TL;DR: It is demonstrated that MAPMT-based MMM has imaging depth comparable to SMM with equivalent sensitivity by imaging tissue phantoms, ex vivo human skin specimens based on endogenous fluorophores, and green fluorescent protein (GFP) expressing neurons in mouse brain slices.

TL;DR: In this article, the authors present a method for imaging tissue, which includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the regions of interest across a portion of tissue, imaging a plurality of layers of the tissue in a plurality volume of the volume of interest and detecting a fluorescence image of tissue due to said excitation light.

TL;DR: In this paper, a multianode photomultiplier tube (MAPMT) is used for high-speed tissue imaging, where the emission photons from the array of excitation foci are collected simultaneously by a MAPMT and the signals from each anode are detected using high sensitivity, low noise single photon counting circuits.

TL;DR: Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution.