Showing papers by "Tojiro Tsushida published in 1995"
TL;DR: It is concluded that quercetin acts as an antioxidant more efficiently than its monoglucosides when phospholipid bilayers are exposed to aqueous oxygen radicals.
155 citations
01 Jan 1995-Journal of The Japanese Society for Food Science and Technology-nippon Shokuhin Kagaku Kogaku Kaishi
TL;DR: The results suggested that the factors which induce the differentiation of U-937 cells into monocytes/macrophages may exist in the non-dialyzable extracts of these vegetables.
Abstract: The effects of non-dialyzable extracts of several vegetables such as eggplant, green pepper, cabbage, Garland chrysanthemum, kidney beans, cucumber and Welsh onion on the differentiation of U-937 human myeloid leukemia cells were examined. Among the vegetables, the extract of eggplant induced the adhesion of U-937 cells on the culture plate and the morphological change of U-937 cells. A few adhesive and elongated cells was detected in the presence of Garland chrysanthemum. The extracts of cucumber and kidney bean induced only the morphological change of U-937 cells. The evident expression of cell surface antigens of CD11b and CD36 of U-937 cells, which is a marker of differentiation of U-937 cells into monocytes/macrophages, was induced by the extracts of eggplant, kidney bean, cucumber and Welsh onion. These results suggested that the factors which induce the differentiation of U-937 cells into monocytes/macrophages may exist in the non-dialyzable extracts of these vegetables.
9 citations
TL;DR: The mutagenicity of extracts from a transgenic tomato that was established by transfection of a gene encoding the coat protein of tobacco mosaic virus to the F1 hybrid between Lycopersicon esculentum LA1000 and L. peruvianum PI128650 was examined by the umu-test with Salmonella typhimurium TA1535/pSK1002 as the test organism.
Abstract: We examined the mutagenicity of extracts (juice and ethanol extract) from a transgenic tomato that was established by transfection of a gene encoding the coat protein of tobacco mosaic virus (TMV) to the F1 hybrid between Lycopersicon esculentum LA1000 and L peruvianum PI128650, by the umu-test with Salmonella typhimurium TA1535/pSK1002 as the test organism The extracts showed no detectable mutagenicity The extracts from the above-mentioned F1 hybrids and wild tomatoes and cultivars (L peruvianum PI128650, L peruvianum PI126944, L pimpinellifolium LS1524, L pimpinellifolium LA722, L hirsutum LS503, Mini-carol, Sun-cherry, Momotaro, Odoriko, Kagome77, and Ponderosa) also showed no detectable mutagenicity
2 citations
01 Jan 1995
TL;DR: Some vegetable extracts and the extracts from miso, a Japanese traditional fermented food, inhibited melanin production of B16 mouse melanoma cells and the use of a protein-free medium improved a sensitivity of the assay.
Abstract: We assessed physiological functions of foodstuffs using cultured mammalian cell lines. Addition of non-dialyzable extracts from several vegetables to U-937 human leukemia cells induced macrophage differentiation markers such as adhesion of the cells, nitro blue tetrazolium-reducing activity and a CD 11 b antigen expression. Some vegetable extracts and the extracts from miso, a Japanese traditional fermented food, inhibited melanin production of B16 mouse melanoma cells. In the assay of an immunoglobulin production using a human-human hybridoma HB4C5, the use of a protein-free medium improved a sensitivity of the assay.
01 Jan 1995
TL;DR: It was shown that gelatin and fibronectin played an important role on the expression of a macrophage function of UM cells, which was established through the differentiation of U-937 human histocytic lymphoma cell line by phorbolester.
Abstract: Serum free culture of the animal cells is an important method of assessing functionalities of food and food components. Recently, we developed an inorganic iron supplemented protein-free medium (Medium920) for hybridoma cells. In this study, we applied the Medium920 and a conventional ERDF medium supplemented with insulin and transferrin (ITES-ERDF) to the serum-free culture of a human macrohagelike cell line (UM) which was established through the differentiation of U-937 human histocytic lymphoma cell line by phorbolester. The UM cells grew well in both media. The growth rate of UM cells in the Medium920 was lower than that in ITESERDF medium, but a long term culture of UM cells in the Medium920 over 2 months was achieved without any changes of the growth rate. From an analysis of a NBT (nitro blue tetrazolium) reducing activity of UM cells cultured in the Medium920 in culture dishes coated with several cell attachment factors, it was shown that gelatin and fibronectin played an important role on the expression of a macrophage function of UM cells.