Tommy Cedervall

TL;DR: The rates of protein association and dissociation are determined using surface plasmon resonance technology with nanoparticles that are thiol-linked to gold, and through size exclusion chromatography of protein–nanoparticle mixtures, and this method is developed into a systematic methodology to isolate nanoparticle-associated proteins.

TL;DR: The long-lived (“hard”) protein corona formed from human plasma is studied for a range of nanoparticles that differ in surface properties and size and both size and surface properties were found to play a very significant role.

TL;DR: The results confirm that significant evolution of the corona occurs in the second biological solution, but that the final corona contains a "fingerprint" of its history, which could be evolved to map the transport pathways utilized by nanoparticles, and eventually to predict nanoparticle fate and behavior.

TL;DR: It is argued that in a biological fluid, proteins associate with nanoparticles, and it is the amount and presentation of the proteins on the surface rather than the particles themselves that are the cause of numerous biological responses.