https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(20)30186-5/pdf

Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay.

Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human coronavirus that can cause human respiratory disease. The development of a detection method for this virus that can lead to rapid and accurate diagnosis would be significant. In this study, we established a nucleic acid visualization technique that combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualization strip (RT-LAMP-VF) to detect the N gene of MERS-CoV. The RT-LAMP-VF assay was performed in a constant temperature water bath for 30 min, and the result was visible by the naked eye within 5 min. The RT-LAMP-VF assay was capable of detecting 2 × 101 copies/μl of synthesized RNA transcript and 1 × 101 copies/μl of MERS-CoV RNA. The method exhibits no cross-reactivities with multiple CoVs including SARS-related (SARSr)-CoV, HKU4, HKU1, OC43 and 229E, and thus exhibits high specificity. Compared to the real-time RT-PCR (rRT-PCR) method recommended by the World Health Organization (WHO), the RT-LAMP-VF assay is easy to handle, does not require expensive equipment and can rapidly complete detection within 35 min.

/pdf/a-rapid-and-specific-assay-for-the-detection-of-mers-cov-3s9af0pshz.pdf

A Rapid and Specific Assay for the Detection of MERS-CoV.

Genetics and genomics of SARS-CoV-2: A review of the literature with the special focus on genetic diversity and SARS-CoV-2 genome detection

Background
Yellow fever (YF) is an acute viral hemorrhagic disease transmitted by Aedes mosquitoes. The causative agent, the yellow fever virus (YFV), is found in tropical and subtropical areas of South America and Africa. Although a vaccine is available since the 1930s, YF still causes thousands of deaths and several outbreaks have recently occurred in Africa. Therefore, rapid and reliable diagnostic methods easy to perform in low-resources settings could have a major impact on early detection of outbreaks and implementation of appropriate response strategies such as vaccination and/or vector control.

/pdf/rapid-molecular-assays-for-the-detection-of-yellow-fever-330hw37gft.pdf

Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings

Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.

Rapid and specific detection of Asian- and African-lineage Zika viruses

A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus.

Methods: We collected 1578 serum samples collected from febrile adults with no evidence of malaria or typhoid exposure visiting Andersen Medical Clinic, Endebess Sub District Hospital and Kitale District Hospital. We used indirect ELISAs to screen for exposure to Dengue, Chikungunya and West Nile Viruses, and Plaque Reduction Neutralisation Tests to confirm the status of those samples that were ELISA positive.

/pdf/seroprevalence-of-chikungunya-west-nile-virus-and-dengue-2asz5aevno.pdf

Toru Kubo

Papers

A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus.

Seroprevalence of Chikungunya, West Nile Virus and Dengue Virus in Febrile Patients visiting selected health facilities in Trans Nzoia County