Showing papers in "Journal of Virological Methods in 2013"

TL;DR: The BCoV RT-RPA constitutes a suitable accurate, sensitive and rapid alternative to the common measures used for bovine coronavirus diagnosis, and the use of a portable fluorescence reading device extends its application potential to use in the field and point-of-care diagnosis.

TL;DR: DNA aptamers that can specifically bind to avian influenza virus (AIV) H5N1 based on Systematic Evolution of Ligands by EXponential enrichment (SELEX) and surface plasmon resonance (SPR) were selected and developed, showing a strong binding between the HA and the selected aptamer.

TL;DR: Silver nanoparticles exhibit remarkably inhibitory effects on adenovirus type 3 in vitro, which suggests silver nanoparticles could be a potential antiviral agent for inhibiting Ad3 infection.

TL;DR: ESMP is an effective low-cost procedure which allows a large number of samples to be processed simultaneously and is easily standardizable for its performance in a routine laboratory working in water monitoring.

TL;DR: Two common methods of quantifying filovirus infectivity, a plaque assay and 50% cell culture infectious dose (TCID50) endpoint dilution assay, were compared and there was a tenfold difference in the numerical results.

TL;DR: Efficient expression of FMDV empty capsids in insect cells after moderation of 3C protease action and efficient empty capsid synthesis may allow development as a vaccine are reported.

TL;DR: A new one-step real-time multiplex RT-PCR that detects and differentiates efficiently WNV-L1, W NV-L2 and USUV in a single reaction that was very sensitive to all of the target viruses.

TL;DR: The GenPospi assay has been shown to be a suitable tool for large-scale screening for all known pospiviroids, and although it has been validated for tomato leaves it can potentially be used for any crop.

TL;DR: Cohen's kappa analysis showed higher than 90% agreement of two commercially available ELISAs with the virus neutralization test, suggesting that primary screening as well as serological confirmation using these ELisAs is feasible.

TL;DR: Serial testing with Stat-Pak as the initial screening test performed as well as parallel testing, but Determine was a less sensitive screen, but Serial testing could be cost saving.

TL;DR: Commercial influenza antigen detection assays are useful tools for the rapid diagnosis of influenza, however, confirmatory testing is always recommended.

TL;DR: The most successful of the four multiplex RT-LAMP assays achieved a diagnostic sensitivity and specificity of 98.0% and 98.1%, and did not falsely detect FMDV in known negatives or samples containing swine vesicle disease virus, vesicular stomatitis virus or vesicles exanthema of swine virus.

TL;DR: Findings indicate that the xCELLigence RTCA system can be a valuable addition to current viral assays for quantitative measurement of infectious viruses and quantitation of neutralization antibody titer in real-time, warranting for future research and exploration of applications to many other animal and human viruses.

TL;DR: The results suggest that the MO BIO kit is appropriate for the extraction and purification of viral nucleic acids from environmental and clinical samples that contain high levels of inhibitors.

TL;DR: This study compares 4 different multiplex PCR assays for the recovery of common respiratory viruses using the xTAG respiratory viral panel fast, Fast-track Respiratory Pathogen assay, Easyplex respiratory pathogen 12 kit, and an in-house multiplex real-time PCR assay.

TL;DR: It is demonstrated that HEV can replicate efficiently in the RWV in human hepatoblastoma PLC/PRF/5 cells for up to 5 months not only by real time RT-PCR but also by detection of complete virions via electron microscopy.

TL;DR: This study investigated the use of deep sequencing by the next-generation sequencing (NGS) Illumina MiSeq Platform to obtain complete genome sequence information from influenza virus isolates to identify seasonal influenza A H3N2, 2009 pandemic influenza AH1N1and influenza B in the DNA library mixtures efficiently.

TL;DR: The loop-mediated isothermal amplification (LAMP) assay for Piper yellow mottle virus and the reverse transcription (RT) LAMP assay for Cucumber mosaic virus each consisted of a set of five primers designed against the conserved sequences in the viral genome.

TL;DR: An antigen-capture enzyme-linked immunosorbent assay employing monoclonal and polyclonal antibodies against p27 detected the virus in the albumin and cloacal swabs of naturally infected chickens and the results were confirmed by PCR, indicating that the AC-ELISA was a suitable method for the detection of ALV.

TL;DR: In this article, a one-step multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) was used to diagnose respiratory infections and has shown to be more sensitive than viral culture and antigen detection.

TL;DR: Modification of the RT-LAMP reactions to use labelled primers allowed rapid detection of amplification products using lateral flow devices containing antibodies specific to the incorporated labels, avoiding the need for fluorescence detection or gel electrophoresis.

TL;DR: This protocol describes a triple-plasmid co-transfection approach with 25 kDa linear polyethylenimine (PEI) in 293 T cells for the production of AAV serotype 2, which showed high expression both in vivo within the brain and in vitro in cell culture.

TL;DR: A decrease in virus infectious ability disclosed by TCID₅₀ indicated that the fraction of non-infectious particles in FCV increased 300,000 times when time 0 and 48 h were compared, which indicated that virus counts need not necessarily correlate withirus infectious ability.

TL;DR: The high prevalence of Grapevine fleck virus, Grapevine leafroll-associated virus 3 and Grapevine fanleaf virus suggests that the main pathways of viral introduction are infected plant material that has escaped controls and/or uncontrolled traffic of propagating plant material.

TL;DR: This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube.

TL;DR: This test offers a simple, rapid and reliable diagnostic tool for yellow fever when there are outbreaks of acute hemorrhagic fever in Kenya and other African countries and increased sensitivity tenfold compared to RT-PCR.

TL;DR: The results indicated that the improved duplex RT-PCR method provides a rapid and cost-effective laboratory differential diagnosis for mixed infection of DHAV-1 and DHAv-3 in ducklings.

TL;DR: MS-1 is an early passage cell line that provides a useful in vitro model to characterize MCV-positive MCC, and Immunophenotypic analysis of the MS-1 cell line and xenografts in mice show identical profiles to the parental tumor biopsy.

TL;DR: Quantitative PCR allows for a superior, rapid, sensitive, and quantitative method for detecting FV3-like virus in box turtles, and this assay will be useful for early detection and disease monitoring.

TL;DR: Multiple methods were explored to determine an appropriate cutoff for a multiplexed microsphere assay that is used to detect henipavirus antibody binding in fruit bat plasma, and this study is presented as an example for others where reference samples, and assays that have been characterised previously, are absent.