Tove M.I.E. Christensen

TL;DR: It is demonstrated that, in addition to blockwise de-esterification, HG with a non-blockwise distribution of methyl esters is also an abundant feature of HG in primary plant cell walls, implications for understanding of both the action of pectin methyl esterases on matrix properties and mechanisms of intercellular adhesion and its loss in plants.

TL;DR: Investigation of the structure of epitopes recognised by anti-pectin monoclonal antibodies found that optimal JIM5 and JIM7 binding occurs where specific but undefined methyl-esterification patterns are present on HG domains, although fully de-esterified HG samples contain sub-optimal J IM5 epitopes.

TL;DR: Introduction of a certain pattern of methyl ester distribution caused by selective removal of certain methyl Ester groups by f-PME is the most reasonable explanation for the detected differences.

TL;DR: New modified pectin have been prepared by treatment of pectins having different DM with NaBH(4) to reduce selectively the methyl esters to primary alcohols in the presence of free acids and exhibit high gelling properties.

TL;DR: Differences between the amounts of galacturonic acid located in exo- and endo-BS, provided evidence for the need of a certain start side or blocklength for p-PME to de-esterify blockwise.