https://helvia.uco.es/xmlui/bitstream/handle/10396/10963/Spectrophotometric%20Quantitation%20%281%29.pdf?sequence=1

Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E.

The antioxidant capacities (oxygen radical absorbance capacity, ORAC) and total phenolic contents in extracts of 27 culinary herbs and 12 medicinal herbs were determined. The ORAC values and total phenolic contents for the medicinal herbs ranged from 1.88 to 22.30 μmol of Trolox equivalents (TE)/g of fresh weight and 0.23 to 2.85 mg of gallic acid equivalents (GAE)/g of fresh weight, respectively. Origanum × majoricum, O. vulgare ssp. hirtum, and Poliomintha longiflora have higher ORAC and phenolic contents as compared to other culinary herbs. The ORAC values and total phenolic content for the culinary herbs ranged from 2.35 to 92.18 μmol of TE/g of fresh weight and 0.26 to 17.51 mg of GAE/g of fresh weight, respectively. These also were much higher than values found in the medicinal herbs. The medicinal herbs with the highest ORAC values were Catharanthus roseus, Thymus vulgaris, Hypericum perforatum, and Artemisia annua. A linear relationship existed between ORAC values and total phenolic contents of th...

Antioxidant activity and phenolic compounds in selected herbs

Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review

Three methods widely employed in the evaluation of antioxidant activity, namely 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and β-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts. The strengths and limitations of each method have been illustrated by testing a number of extracts, of differing polarity, from plants of the genus Sideritis, and two known antioxidants (butylated hydroxytoluene and rosmarinic acid). The sample polarity was important for the exhibited activity in the BCBT and HS-GC methods but not for the DPPH method. The complex composition of the extracts and partition phenomena affected their activity in each assay. The value of the BCBT method appears to be limited to less polar samples. Although slow, the HS-GC method is preferable for assessing the antioxidant inhibitory properties on the formation of unwanted secondary volatile products. Being rapid, simple and independent of sample polarity, the DPPH method is very convenient for the quick screening of many samples for radical scavenging activity. Copyright © 2001 John Wiley & Sons, Ltd.

Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods

Deep-fat frying produces desirable or undesirable flavor compounds and changes the flavor stability and quality of the oil by hydrolysis, oxidation, and polymerization. Tocopherols, essential amino acids, and fatty acids in foods are degraded during deep-fat frying. The reactions in deep-fat frying depend on factors such as replenishment of fresh oil, frying conditions, original quality of frying oil, food materials, type of fryer, antioxidants, and oxygen concentration. High frying temperature, the number of fryings, the contents of free fatty acids, polyvalent metals, and unsaturated fatty acids of oil decrease the oxidative stability and flavor quality of oil. Antioxidant decreases the frying oil oxidation, but the effectiveness of antioxidant decreases with high frying temperature. Lignan compounds in sesame oil are effective antioxidants in deep-fat frying.

https://onlinelibrary.wiley.com:443/doi/pdf/10.1111/j.1750-3841.2007.00352.x

Chemistry of deep-fat frying oils

The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75°C. The concentration of extracts in lard varied from 0.01 to 0.20%. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. The induction period of lard increased with antioxidant concentration. After the induction period, peroxide formation proceeded rapidly, following pseudo-zero order reaction kinetics. The rate of the reaction decreased slightly with increasing plant extract concentration. Combined addition of plant extracts in lard showed a low synergistic action between thyme extract and spearmint extract.

Antioxidant activity of some plant extracts of the family labiatae

HPLC-DAD coupled with mass spectrometry in the positive ionization mode was applied to study the fragmentation of twelve selected flavonoids. Compounds belonging to all the major subgroups found in common plants, i.e. flavonols, flavones, dihydroflavonols, flavanones and flavanols were studied. Compound standards were injected into the spectrometer and produced characteristic mass spectra. The fragmentation of each compound was studied and it was shown that the dehydration and carbon monoxide losses from the [M+H]+ ion by the members of each subgroup produced specific fragments, thus allowing the characterization of the flavonoid subgroups. Moreover, fragments resulting from fission of the C-rings are specific of each subgroup and revealed the substitution pattern of A- and B-rings. In order to verify the identifying efficiency of the positive ionization mode through these characteristic fragmentations, the unknown flavonoids of an Origanum vulgare diethyl ether extract were separated with the HPLC system and the major peaks were successfully identified with the mass spectrometer.

/pdf/characterization-of-flavonoid-subgroups-and-hydroxy-4s7q61lyn0.pdf

Characterization of Flavonoid Subgroups and Hydroxy Substitution by HPLC-MS/MS

Water loss and oil uptake as a function of frying time

Background Lipid oxidation is one of the main causes of food spoilage. It can be delayed by the addition of antioxidants to food or by using vacuum or modified atmosphere packaging. A recent approach is the application of antioxidants in active packaging. Scope and approach The scope of this review is to present and update all information about the incorporation of natural antioxidants in edible and active films and coatings. Natural antioxidants can replace chemical additives and are preferably accepted by the consumers. Their effect on the barrier, mechanical and antioxidant properties of the various films (chitosan, cellulose derivatives gelatin etc.) are discussed. The ability to delay food oxidation is examined in relation to the active components. Key findings and conclusions Water or ethanol extracts from several herbs (rosemary, oregano, tea), berries, or plant by-products increased the total phenolic content and the antiradical activity of the films and proved efficient in delaying the oxidation of fish and meat products. Also, a protective activity was observed from the essential oils that were rich in carvacrol or thymol. The barrier properties of the films were positively affected, due to interactions between the active groups of the additive (e.g. polyphenols) and the film matrix. On the contrary, hydrophilic additives increased water vapor and oxygen permeability. The mechanical properties were in most cases adversely affected. Results are promising and further research on the release of antioxidant compound into food, the effect on sensorial properties, and application to other lipid foods may encourage practical application.

Edible and active films and coatings as carriers of natural antioxidants for lipid food

Tomato waste is an important source of natural carotenoids This study was carried out to assess the extractability of tomato waste carotenoids in different organic solvents and to optimise the extraction parameters (type of solvent, extraction time, temperature and extraction steps) for maximum yield Among other solvents, we tested a new environmentally friendly one, ethyl lactate, which gave the highest carotenoid yield (24300 mg kg -1 dry tomato waste) at 70 °C, compared to acetone (5190 mg kg -1 ), ethyl acetate (4621 mg kg -1 ), hexane (3445 mg kg -1 ) and ethanol (1757 mg kg -1 ) The carotenoid recovery was significantly (P < 005) affected by the number of extraction steps and temperature in all solvents Mathematic equations predicted rather satisfactorily (R 2 = 089-093) the rate of carotenoid extraction in the above-mentioned solvents Carotenoid concentration increased with time, approaching a quasi-saturated condition at approximately 30 min of extraction

Vassiliki Oreopoulou

Papers

Antioxidant activity of some plant extracts of the family labiatae

Characterization of Flavonoid Subgroups and Hydroxy Substitution by HPLC-MS/MS

Water loss and oil uptake as a function of frying time

Edible and active films and coatings as carriers of natural antioxidants for lipid food

Effect of extraction parameters on the carotenoid recovery from tomato waste