V
Victoria L. Singer
Researcher at Molecular Probes
Publications - 32
Citations - 4211
Victoria L. Singer is an academic researcher from Molecular Probes. The author has contributed to research in topics: Staining & Fluorescence. The author has an hindex of 25, co-authored 32 publications receiving 4046 citations. Previous affiliations of Victoria L. Singer include University of California, Berkeley.
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Journal ArticleDOI
Characterization of PicoGreen Reagent and Development of a Fluorescence-Based Solution Assay for Double-Stranded DNA Quantitation
TL;DR: The PicoGreen assay allowed the detection of 25 pg/ml ds DNA, surpassing the sensitivity achieved with Hoechst 33258 by 400-fold, and showed greater dsDNA:RNA selectivity than HoeChSt 33258 in low ionic strength buffer and better dSDNA:single-stranded DNA selectivity in 1 M NaCl.
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Analysis of mitochondrial morphology and function with novel fixable fluorescent stains.
Martin Poot,Yu-zhong Zhang,J. A. Kramer,K S Wells,L J Jones,D K Hanzel,A G Lugade,Victoria L. Singer,Rosaria P. Haugland +8 more
TL;DR: CMXRos has the potential to monitor changes in mitochondrial function and was used in conjunction with spectrally distinct fluorescent probes for the cell nucleus and the microtubule network to concomitantly evaluate multiple features of cell morphology.
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Characterization of SYBR Gold Nucleic Acid Gel Stain: A Dye Optimized for Use with 300-nm Ultraviolet Transilluminators
Rabiya S. Tuma,Matthew P. Beaudet,Xiaokui Jin,Laurie J. Jones,Ching-Ying Cheung,Stephen T. Yue,Victoria L. Singer +6 more
TL;DR: This new unsymmetrical cyanine dye, SYBR Gold nucleic acid gel stain, has two fluorescence excitation maxima when bound to DNA, one centered at approximately 300 nm and one at approximately 495 nm, and is found to be as sensitive as silver staining for detecting DNA-with a single-step staining procedure.
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SYPRO orange and SYPRO red protein gel stains: one-step fluorescent staining of denaturing gels for detection of nanogram levels of protein
TL;DR: Two new fluorescent dyes are developed, SYPRO Orange protein gel stain and SYPRO Redprotein gel stain, to detect proteins in electrophoretic gels, allowing multiple photographs of gels to be taken without significant signal reduction.
Journal ArticleDOI
RNA Quantitation by Fluorescence-Based Solution Assay: RiboGreen Reagent Characterization
TL;DR: A sensitive fluorescence-based solution assay for RNA using a new dye, RiboGreen RNA quantitation reagent, which exhibits >1000-fold fluorescence enhancement and high quantum yield upon binding nucleic acids, with excitation and emission maxima near those of fluorescein.